Department of Engineering, Air Quality Engineering, Aarhus University, Finlandsgade 10, 8200 Aarhus N, Denmark.
Department of Chemical Engineering, Biotechnology and Environmental Technology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark.
Environ Sci Technol. 2020 Jun 16;54(12):7639-7650. doi: 10.1021/acs.est.0c01231. Epub 2020 Jun 3.
Gaseous emissions from livestock production are complex mixtures including ammonia, methane, volatile organic compounds (VOC), and HS. These contribute to eutrophication, reduced air quality, global warming, and odor nuisance. It is imperative that these gases are mitigated in an environmentally sustainable manner. We present the discovery of a microbial inhibitor combo consisting of tannic acid and sodium fluoride (TA-NaF), which exhibits clear synergistic inhibition of ammonia production in pure bacteria culture and in pig manure while simultaneously inhibiting methane and odorant (HS and VOC) emissions. In laboratory headspace experiments on pig manure, we used proton-transfer-reaction mass spectrometry and cavity ring-down spectroscopy to measure the effect of TA-NaF on gaseous emissions. Ammonia emission was reduced by more than 95%, methane by up to ∼99%, and odor activity value by more than 50%. Microbial community analysis and gas emission data suggest that TA-NaF acts as an efficient generic microbial inhibitor, and we hypothesize that the synergistic inhibitory effect on ammonia production is related to tannic acid causing cell membrane leakage allowing fluoride ions easy access to urease.
畜牧业产生的气体排放物是复杂的混合物，包括氨、甲烷、挥发性有机化合物（VOC）和 H2S。这些物质会导致富营养化、空气质量下降、全球变暖以及恶臭滋扰。因此，必须以环境可持续的方式来减轻这些气体的排放。我们发现了一种由单宁酸和氟化钠组成的微生物抑制剂组合（TA-NaF），它在纯细菌培养物和猪粪中对氨的产生表现出明显的协同抑制作用，同时抑制甲烷和有气味的（H2S 和 VOC）排放物。在猪粪的实验室顶空实验中，我们使用质子转移反应质谱和腔衰荡光谱来测量 TA-NaF 对气体排放的影响。氨排放减少了 95%以上，甲烷减少了 99%左右，气味活性值减少了 50%以上。微生物群落分析和气体排放数据表明，TA-NaF 是一种有效的通用微生物抑制剂，我们假设其对氨产生的协同抑制作用与单宁酸导致细胞膜渗漏有关，使氟离子容易接触到脲酶。
