基于 ESIPT 的荧光探针的合理设计，用于选择性监测活细胞和斑马鱼中的谷胱甘肽。
Rational design of an ESIPT-based fluorescent probe for selectively monitoring glutathione in live cells and zebrafish.
机构信息
State Key Laboratory of Applied Organic Chemistry, Lanzhou University, 222 Tianshui Street S., Lanzhou, Gansu 730000, China.
State Key Laboratory of Applied Organic Chemistry, Lanzhou University, 222 Tianshui Street S., Lanzhou, Gansu 730000, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Sep 5;238:118429. doi: 10.1016/j.saa.2020.118429. Epub 2020 Apr 28.
Glutathione (GSH), an extremely important antioxidant, is a major participant in maintaining redox homeostasis and tightly associated with various clinical diseases. Thus, accurate and rapid detection of intracellular GSH is imperative to elucidate its role in physiological and pathological processes. Herein, by modifying 2-(2'-hydroxyphenyl) benzothiazole (HBT) scaffold, we developed an excited-state intramolecular proton transfer (ESIPT)-based fluorescent probe BTFMD for tracking GSH, which exhibited good selectivity, excellent water solubility, a large Stokes shift (181 nm) and fast response rate (within 10 min). Furthermore, the probe was successfully applied for imaging of endogenous GSH in live cells and zebrafish, and probing into the role of GSH in the development of cancer and Parkinson's disease.
谷胱甘肽（GSH）是一种极其重要的抗氧化剂，是维持氧化还原平衡的主要参与者，与各种临床疾病密切相关。因此，准确快速地检测细胞内 GSH 对于阐明其在生理和病理过程中的作用至关重要。在此，我们通过修饰 2-(2'-羟基苯基)苯并噻唑（HBT）骨架，开发了一种基于激发态分子内质子转移（ESIPT）的荧光探针 BTFMD 来跟踪 GSH，该探针具有良好的选择性、优异的水溶性、大的斯托克斯位移（181nm）和快速的响应速度（在 10 分钟内）。此外，该探针成功地应用于活细胞和斑马鱼内源性 GSH 的成像，并探究了 GSH 在癌症和帕金森病发展中的作用。
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