Department of Science and Environment, Enhanced Perioperative Oncology (EPeOnc) Consortium, Roskilde University, Universitetsvej 1, 4000, Roskilde, Denmark.
Center for Surgical Science, Enhanced Perioperative Oncology (EPeOnc) Consortium, Department of Surgery, Zealand University Hospital, Lykkebækvej 1, 4600, Køge, Denmark.
BMC Cancer. 2020 May 14;20(1):426. doi: 10.1186/s12885-020-06941-y.
Colon cancer is one of the most commonly diagnosed types of cancer with surgical resection of the tumor being the primary choice of treatment. However, the surgical stress response induced during treatment may be related to a higher risk of recurrence. The aim of this study was to examine the effect of surgery on adhesion of cultured colon cancer cells with or without expression of the tumour suppressor CDX2.
We enrolled 30 patients undergoing elective, curatively intended laparoscopic surgery for colon cancer in this study. Blood samples were drawn 1 day prior to surgery and 24 h after surgery. The samples of pre- and postoperative serum was applied to wild type colon cancer LS174T cells and CDX2 inducible LS174T cells and adhesion was measured with Real-Time Cell-Analysis iCELLigence using electrical impedance as a readout to monitor changes in the cellular adhesion.
Adhesion abilities of wild type LS174T cells seeded in postoperative serum was significantly increased compared to cells seeded in preoperative serum. When seeding the CDX2 inducible LS174T cells without CDX2 expression in pre- and postoperative serum, no significant difference in adhesion was found. However, when inducing CDX2 expression in these cells, the adhesion abilities in pre- and postoperative serum resembled those of the LS174T wild type cell line.
We found that the adhesion of colon cancer cells was significantly increased in postoperative versus preoperative serum, and that CDX2 expression affected the adhesive ability of cancer cells. The results of this study may help to elucidate the pro-metastatic mechanisms in the perioperative phase and the role of CDX2 in colon cancer metastasis.
结肠癌是最常见的癌症类型之一，肿瘤切除术是主要的治疗选择。然而，治疗过程中引起的手术应激反应可能与更高的复发风险有关。本研究旨在研究手术对表达肿瘤抑制因子 CDX2 的培养结肠癌细胞黏附的影响。
本研究纳入 30 例接受择期、根治性腹腔镜结肠癌手术的患者。手术前 1 天和手术后 24 小时抽取血样。将术前和术后血清样本应用于野生型结肠癌细胞 LS174T 和诱导型 CDX2 的 LS174T 细胞，并使用实时细胞分析 iCELLigence 通过电阻抗作为读出值测量细胞黏附的变化来测量黏附能力。
与术前血清相比，接种在术后血清中的野生型 LS174T 细胞的黏附能力显著增加。当在术前和术后血清中接种不表达 CDX2 的诱导型 CDX2 的 LS174T 细胞时，黏附没有明显差异。然而，当诱导这些细胞中 CDX2 的表达时，预、术后血清中的黏附能力与 LS174T 野生型细胞系相似。
我们发现结肠癌细胞在术后血清中的黏附能力明显高于术前血清，且 CDX2 表达影响癌细胞的黏附能力。本研究的结果可能有助于阐明围手术期的促转移机制以及 CDX2 在结肠癌转移中的作用。
