Department of General Surgery, First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, People's Republic of China.
Pathol Oncol Res. 2011 Sep;17(3):743-51. doi: 10.1007/s12253-011-9380-0. Epub 2011 Apr 7.
The homeobox gene, CDX2, plays a major role in development, especially in the gut, and also functions as a tumor suppressor in the adult colon. In the present study, we investigated the effects of CDX2 expression on the proliferation, migration, and apoptosis of the human colon cancer cell line, Lovo. Lovo cells exogenously expressing CDX2 exhibited no significant differences in the percentage of cells in G1- and S-phase or in apoptosis, as determined by flow cytometry. MTT assay also confirmed that CDX2 expression had no effect on proliferation in these cells. Interestingly, conditioned medium collected from CDX2-overexpressing Lovo cells showed a significant decrease in secretion of MMP-2 and the invasive potential of these cells was significantly inhibited. Collectively, these data suggest that CDX2 may play a critical role in the migration and metastasis of colon carcinoma and over-expression of CDX2 in colon cancer cells markedly inhibits invasion. Based on these results, exogenous expression of CDX2 might be a promising option in the treatment of colon carcinoma.
同源盒基因 CDX2 在发育过程中起着重要作用，特别是在肠道中，并且在成人结肠中作为肿瘤抑制因子发挥作用。在本研究中，我们研究了 CDX2 表达对人结肠癌细胞系 Lovo 的增殖、迁移和凋亡的影响。通过流式细胞术检测，外源性表达 CDX2 的 Lovo 细胞在 G1 期和 S 期细胞的百分比或细胞凋亡方面没有显著差异。MTT 试验也证实 CDX2 表达对这些细胞的增殖没有影响。有趣的是，从过表达 CDX2 的 Lovo 细胞中收集的条件培养基显示 MMP-2 的分泌显著减少，并且这些细胞的侵袭能力显著受到抑制。综上所述，这些数据表明 CDX2 可能在结肠癌的迁移和转移中发挥关键作用，并且结肠癌中 CDX2 的过表达明显抑制了侵袭。基于这些结果，CDX2 的外源性表达可能是治疗结肠癌的一种有前途的选择。
