Gottscho A M, Lin J L, Duck W N, Losty T A
Helme Tobacco Co., Lancaster, PA 17603.
J Assoc Off Anal Chem. 1988 Nov-Dec;71(6):1110-5.
The use of AOAC standard methods for the determination of nicotine in commercial tobacco products is shown to be inappropriate. The methods do not provide for suitable sample preparation of these products for the analysis and, in addition, they yield high results. Evidence is presented that the nicotine or total alkaloids measured includes artifact nicotine. The distillation method was developed at a time when the complex alkaloid chemistry of tobacco was unknown, and does not take into consideration the possible contribution of substances in tobacco capable of contributing falsely to the result. The Cundiff-Markunas method, developed more recently, also fails to account for this effect--probably because it was designed to produce results matching those of the distillation method. Replacement of the standard methods is proposed and some alternative procedures are suggested.
结果表明，采用美国官方分析化学师协会（AOAC）的标准方法测定商业烟草制品中的尼古丁并不合适。这些方法无法为分析这些产品提供合适的样品制备，此外，它们得出的结果偏高。有证据表明，所测得的尼古丁或总生物碱中包含人为产生的尼古丁。蒸馏法是在烟草复杂的生物碱化学性质尚不明确时开发的，没有考虑到烟草中可能会对结果产生错误影响的物质的潜在作用。最近开发的坎迪夫 - 马库纳斯（Cundiff-Markunas）方法也未能考虑到这种影响——可能是因为它旨在得出与蒸馏法相匹配的结果。本文提议更换标准方法，并提出了一些替代程序。
