Felice L, Abdennebi E H, Ashraf M
University of Minnesota, College of Veterinary Medicine, Department of Veterinary Diagnostic Investigation, St. Paul, 55108.
J Assoc Off Anal Chem. 1988 Nov-Dec;71(6):1156-7.
A liquid chromatographic method is described for the measurement of thiamphenicol in bovine plasma. The plasma (1 mL) is extracted with ethyl acetate. After the solvent is evaporated under a stream of nitrogen, the residue is reconstituted in methanol-water and analyzed by reverse-phase liquid chromatography with UV detection at 224 nm. The intra-day recoveries for bovine plasma spiked with 5 and 50 micrograms/mL of thiamphenicol were 102 and 101%, respectively, with coefficients of variation of 2.40 and 0.28%, respectively. The interday recoveries for the 5 and 50 micrograms/mL samples were 103 and 101%, respectively, with coefficients of variation of 3.40 and 0.94%, respectively. The sensitivity of the method allows quantitation to at least the 100 ng/mL level.
描述了一种用于测定牛血浆中氟苯尼考的液相色谱方法。取1 mL血浆用乙酸乙酯萃取。在氮气流下蒸发溶剂后，将残渣用甲醇 - 水复溶，并通过反相液相色谱在224 nm处进行紫外检测分析。添加了5和50微克/毫升氟苯尼考的牛血浆日内回收率分别为102%和101%，变异系数分别为2.40%和0.28%。5和50微克/毫升样品的日间回收率分别为103%和101%，变异系数分别为3.40%和0.94%。该方法的灵敏度允许至少定量至100纳克/毫升水平。
