Department of Developmental Biology and Department of Genetics, Washington University in St. Louis, St. Louis, MO, USA.
Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO, USA.
Methods Mol Biol. 2020;2144:29-45. doi: 10.1007/978-1-0716-0592-9_3.
We miniaturized standard, solid-phase C. elegans culture conditions to produce a system in which many isolated, individual C. elegans can be housed throughout their lives. This system, the "worm corral," is compatible with high-resolution brightfield and fluorescent microscopy, allowing imaging of fluorescent transgenes and morphological phenotypes from hatch until death. These culture devices can be constructed on the benchtop with commercially available reagents and standard laboratory equipment, making this an attainable solution for most labs.
我们将标准的、固态秀丽隐杆线虫培养条件微型化，从而开发出一种系统，该系统可以在整个生命周期中容纳许多单独的秀丽隐杆线虫。这个系统，即“虫笼”，与高分辨率明场和荧光显微镜兼容，允许从孵化到死亡对荧光转基因和形态表型进行成像。这些培养装置可以使用市售试剂和标准实验室设备在实验台上构建，因此对于大多数实验室来说，这是一种可行的解决方案。
