秀丽隐杆线虫肠道微生物组变化的高通量评估

High-Throughput Assessment of Changes in the Caenorhabditis elegans Gut Microbiome.

作者信息

Zhang Fan, Weckhorst Jessica L, Assié Adrien, Khodakova Anastasia S, Loeza-Cabrera Mario, Vidal Daniela, Samuel Buck S

机构信息

Alkek Center for Metagenomics and Microbiome Research, Baylor College of Medicine, Houston, TX, USA.

出版信息

Methods Mol Biol. 2020;2144:131-144. doi: 10.1007/978-1-0716-0592-9_12.

DOI:10.1007/978-1-0716-0592-9_12

PMID:32410031

Abstract

The gut microbiome is an important driver of host physiology and development. Altered abundance or membership of this microbe community can influence host health and disease progression, including the determination of host lifespan and healthspan. Here, we describe a robust pipeline to measure microbiome abundance and composition in the C. elegans gut that can be applied to examine the role of the microbiome on host aging or other physiologic processes.

摘要

肠道微生物群是宿主生理和发育的重要驱动因素。这个微生物群落的丰度或组成发生改变会影响宿主健康和疾病进展，包括决定宿主的寿命和健康跨度。在这里，我们描述了一种强大的方法来测量秀丽隐杆线虫肠道中的微生物群丰度和组成，该方法可用于研究微生物群在宿主衰老或其他生理过程中的作用。