Winterbourn Christine C
Centre for Free Radical Research, Department of Pathology and Biomedical Science, University of Otago Christchurch, Christchurch, New Zealand.
Biochem Soc Trans. 2020 Jun 30;48(3):745-754. doi: 10.1042/BST20190049.
Reversible oxidation of thiol proteins is an important cell signalling mechanism. In many cases, this involves generation or exposure of the cells to H2O2, and oxidation of proteins that are not particularly H2O2-reactive. There is a conundrum as to how these proteins are oxidised when other highly reactive proteins such as peroxiredoxins are present. This article discusses potential mechanisms, focussing on recent evidence for oxidation being localised within the cell, redox relays involving peroxiredoxins operating in some signalling pathways, and mechanisms for facilitated or directed oxidation of specific targets. These findings help define conditions that enable redox signalling but there is still much to learn regarding mechanisms.
硫醇蛋白的可逆氧化是一种重要的细胞信号传导机制。在许多情况下，这涉及细胞产生或暴露于过氧化氢，以及对并非特别具有过氧化氢反应性的蛋白质进行氧化。当存在诸如过氧化物酶等其他高反应性蛋白质时，这些蛋白质如何被氧化是一个难题。本文讨论了潜在机制，重点关注氧化定位于细胞内的最新证据、涉及在某些信号通路中起作用的过氧化物酶的氧化还原中继，以及特定靶标的促进或定向氧化机制。这些发现有助于确定实现氧化还原信号传导的条件，但在机制方面仍有许多需要了解的地方。
