Lyall F, Morton J J, Lever A F, Cragoe E J
MRC Blood Pressure Unit, Western Infirmary, Glasgow, UK.
J Hypertens Suppl. 1988 Dec;6(4):S438-41. doi: 10.1097/00004872-198812040-00138.
Angiotensin II (Ang II) stimulated the growth of cultured vascular smooth muscle cells in a dose-dependent manner; a small but significant increase being seen at 10(-9) mol/l, whereas 10(-5) mol/l produced a 166% increase compared with control cells. Saralasin blocked the Ang II-mediated increase in cell number. Amiloride-sensitive Na+-H+ exchange was characterized (Km = 18 mmol/l, Vmax = 0.27 pH units/30 s) in cell monolayers using the fluorescent probe biscarboxyethylcarboxy fluorescein. Angiotensin II (10(-7) mol/l) produced a small overall net intracellular alkalinization. Raising intracellular pH via Na+-H+ activation might be one of the ways in which Ang II increases cell growth.
血管紧张素II（Ang II）以剂量依赖的方式刺激培养的血管平滑肌细胞生长；在10^(-9)摩尔/升时可见小幅但显著的增加，而与对照细胞相比，10^(-5)摩尔/升时增加了166%。沙拉新阻断了Ang II介导的细胞数量增加。使用荧光探针双羧乙基羧基荧光素对细胞单层中的氨氯地平敏感的Na^+-H^+交换进行了表征（Km = 18毫摩尔/升，Vmax = 0.27 pH单位/30秒）。血管紧张素II（10^(-7)摩尔/升）导致总体细胞内小幅碱化。通过Na^+-H^+激活提高细胞内pH值可能是Ang II增加细胞生长的方式之一。
