Dai H, Liao H J, Chiang K S
Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan, Republic of China.
Microbios. 1988;56(228-229):157-67.
Stability of carrier state in filamentous phage-infected Xanthomonas campestris pv citri varied drastically even for closely related phage types. The spontaneous curing frequency for cells infected with Cf16-12, Cf16, Cf16-v1 and Cf was 1, 5, 96 and 100%, respectively. The size of the phage replicative-form (RF) pool which built up rapidly at the onset of Cf16 infection was critical to the maintenance of the carrier state and the eventual integration of the prophage. A correlation in stability between phage carrier state in infected cells and lysogeny was found for these phages. The past history of a phage infection altered the response of the cured host to reinfection. In those cured cells which remained susceptible, the stability of the reinfecting phage genome was not altered appreciably, while a distinctive new plaque morphology appeared in greater than 50% of these cells. These alterations were not dependent on the prophage integration prior to curing, and no phage DNA was detected in cured cells by blot hybridisation. Notwithstanding these changes, the sites of phage integration in cured cells remained the same as the original infection on both the phage RF and the host chromosome.
即使对于亲缘关系密切的噬菌体类型，丝状噬菌体感染的野油菜黄单胞菌柑橘致病变种中载体状态的稳定性也有很大差异。感染Cf16 - 12、Cf16、Cf16 - v1和Cf的细胞的自发治愈频率分别为1%、5%、96%和100%。在Cf16感染开始时迅速积累的噬菌体复制型（RF）库的大小对于载体状态的维持和原噬菌体的最终整合至关重要。对于这些噬菌体，发现感染细胞中的噬菌体载体状态与溶原性之间存在稳定性相关性。噬菌体感染的既往史改变了治愈宿主对再感染的反应。在那些仍易感的治愈细胞中，再感染噬菌体基因组的稳定性没有明显改变，而在超过50%的这些细胞中出现了独特的新噬菌斑形态。这些改变不依赖于治愈前的原噬菌体整合，通过印迹杂交在治愈细胞中未检测到噬菌体DNA。尽管有这些变化，治愈细胞中噬菌体整合位点在噬菌体RF和宿主染色体上仍与原始感染位点相同。
