Paschen W, Röhn G, Hallmayer J, Mies G
Department of Experimental Neurology, Max-Planck-Institute for Neurological Research, FRG.
Metab Brain Dis. 1988 Dec;3(4):297-302. doi: 10.1007/BF00999541.
Reversible cerebral ischemia was produced in Mongolian gerbils by occluding both common carotid arteries. Following 5 min of ischemia brains were recirculated for 8, 24, or 96 hr. At the end of the experiments tissue samples were taken from the cerebral cortex and CA1 subfield of the hippocampus for measuring putrescine content and ornithine decarboxylase (ODC) activity. In 5 of 10 animals subjected to 96 hr of recirculation pentobarbital (50 mg/kg) was injected during early recirculation, and the density of ischemic cell damage was determined in the CA1 subfield of the hippocampus in treated and untreated animals. Reversible cerebral ischemia induced a drastic increase in ODC activity after 8 hr of recirculation (about 14-fold in the cortex and 7-fold in the hippocampus), which was markedly reduced following 24 hr of recirculation. Putrescine, in contrast, was high following 8 hr of recirculation and increased even further from 8 to 24 hr of recirculation. Postischemic pentobarbital treatment of animals significantly reduced both the increase in putrescine and the density of ischemic cell damage in the hippocampus. The results are discussed in view of the known activities of putrescine.
通过阻断双侧颈总动脉，在蒙古沙鼠中造成可逆性脑缺血。缺血5分钟后，使大脑再灌注8、24或96小时。实验结束时，从大脑皮层和海马体CA1亚区采集组织样本，以测量腐胺含量和鸟氨酸脱羧酶（ODC）活性。在10只接受96小时再灌注的动物中，有5只在再灌注早期注射了戊巴比妥（50mg/kg），并测定了治疗组和未治疗组动物海马体CA1亚区缺血性细胞损伤的密度。可逆性脑缺血在再灌注8小时后导致ODC活性急剧增加（皮层中约增加14倍，海马体中增加7倍），在再灌注24小时后显著降低。相比之下，腐胺在再灌注8小时后含量较高，从再灌注8小时到24小时进一步增加。对动物进行缺血后戊巴比妥治疗可显著降低腐胺的增加以及海马体中缺血性细胞损伤的密度。根据腐胺的已知活性对结果进行了讨论。
