通过 Western blot 提高转录因子 C/EBP 同源蛋白的免疫检测
Improvement of immunodetection of the transcription factor C/EBP homologous protein by western blot.
机构信息
Université de La Réunion, INSERM UMR 1187, CNRS 9192, IRD 249, Processus Infectieux en Milieu Insulaire Tropical (PIMIT), Plateforme CYROI, 2, Rue Maxime Rivière, 97490, Sainte-Clotilde, Ile de La Réunion, France.
Université de La Réunion, INSERM UMR 1188, Diabète Athérothrombose Thérapies Réunion Océan Indien (DéTROI), Plateforme CYROI, 2, Rue Maxime Rivière, 97490, Sainte-Clotilde, Ile de La Réunion, France; CHU de La Réunion, Saint-Denis, France.
出版信息
Anal Biochem. 2020 Jul 15;601:113775. doi: 10.1016/j.ab.2020.113775. Epub 2020 May 13.
Accumulation of misfolded proteins within the endoplasmic reticulum (ER) induces an unfolded protein response (UPR) that either restores homeostasis or triggers apoptosis in case of adaptation failure. The three activated branches of UPR lead to IRE1-, PERK- and ATF6- dependent transcriptional induction of the gene encoding the transcription factor C/EBP homologous protein (CHOP) which plays an important role in apoptosis induction. In conventional immunoblotting conditions, detection of CHOP is a difficult task. Using a fixation step, we have optimized the detection of CHOP and this method provides a valuable tool to decipher CHOP involvement in UPR.
内质网（ER）中错误折叠蛋白的积累会诱导未折叠蛋白反应（UPR），如果适应失败，该反应要么恢复体内平衡，要么引发细胞凋亡。UPR 的三条激活分支导致IRE1、PERK 和 ATF6 依赖性转录诱导编码转录因子 C/EBP 同源蛋白（CHOP）的基因，CHOP 在凋亡诱导中发挥重要作用。在常规免疫印迹条件下，CHOP 的检测是一项艰巨的任务。通过固定步骤，我们优化了 CHOP 的检测，该方法为破译 CHOP 在 UPR 中的作用提供了有价值的工具。
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