College of Food Science & Technology, Shanghai Ocean University, Shanghai, China.
College of Food Science & Technology, Shanghai Ocean University, Shanghai, China; Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai, China; Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai, China; Department of Food Science, Foshan University, Foshan, 528000, China.
Sci Total Environ. 2020 Aug 20;731:138386. doi: 10.1016/j.scitotenv.2020.138386. Epub 2020 May 6.
Vibrio parahaemolyticus is a kind of gram-negative marine pathogen, which usually adheres to stainless steel (SS), glass (GS) and other abiotic surfaces in aquaculture and food processing in the form of biofilm and causes the spread of gastrointestinal illness. However, the deeply survival adaptation mechanism of V. parahaemolyticus biofilm cells on these contact surface remained unclear. Here, proteomics was used to investigated the physiological response of the V. parahaemolyticus biofilms cells to different abiotic surfaces (SS, GS and polystyrene (PS)). In addition, the effect of contact materials on the physical-chemical properties of biofilms are also characterized. Results showed that the expression of proteins of biofilm cells established on the SS surface were mainly related to the alleviation of metal ion stress and toxicity. The up-regulated proteins in the biofilm cells formed on the GS surface were mainly involved in the biological processes of sugar uptake, protein synthesis and bacterial chemotaxis. Meanwhile, the significantly expressed proteins in the biofilm cells formed on the PS surface were mainly involved in the cellular physiological activity of aromatic compound metabolism, osmotic stress and nutrient transport. All functional proteins mentioned above were closely related to the interaction characteristics of the contact surface and biofilm. This study provided an in-depth comparison of V. parahaemolyticus biofilm formation on these three abiotic surfaces, and presented a model in first time for the adaptation behavior of biofilm cells on different surfaces as affected by metal ion stress, nutrition, osmotic stress, and sugar utilization, which could facilitate an efficient control strategy for biofilm formation in industrial field.
副溶血性弧菌是一种革兰氏阴性海洋病原体，通常以生物膜的形式附着在水产养殖和食品加工中的不锈钢（SS）、玻璃（GS）和其他非生物表面上，并导致胃肠道疾病的传播。然而，副溶血性弧菌生物膜细胞在这些接触表面上的深度生存适应机制仍不清楚。在这里，蛋白质组学被用于研究副溶血性弧菌生物膜细胞对不同非生物表面（SS、GS 和聚苯乙烯（PS））的生理反应。此外，还对接触材料对生物膜物理化学性质的影响进行了特征描述。结果表明，在 SS 表面形成的生物膜细胞中表达的蛋白质主要与缓解金属离子应激和毒性有关。在 GS 表面形成的生物膜细胞中上调的蛋白质主要参与糖摄取、蛋白质合成和细菌趋化性等生物过程。同时，在 PS 表面形成的生物膜细胞中显著表达的蛋白质主要参与芳香族化合物代谢、渗透胁迫和营养物质运输等细胞生理活性。上述所有功能蛋白都与接触表面和生物膜的相互作用特性密切相关。本研究深入比较了副溶血性弧菌在这三种非生物表面上的生物膜形成，首次提出了生物膜细胞在不同表面上受金属离子胁迫、营养、渗透压和糖利用影响的适应行为模型，为工业领域生物膜形成的有效控制策略提供了依据。
