Laboratory of Microbiology and Technology of Marine Products (MICROTEC), Instituto de Investigaciones Marinas (IIM-CSIC), Eduardo Cabello, 6, 36208 Vigo, Pontevedra, Spain.
Department of Chemical Engineering, Faculty of Science, University of Vigo (Campus Ourense), As Lagoas, 32004 Ourense, Spain.
Sci Total Environ. 2020 Aug 15;730:139143. doi: 10.1016/j.scitotenv.2020.139143. Epub 2020 May 4.
A biorefinery scheme for the valorization of horse chestnut biowastes (a municipal solid waste) into added value bioactive compounds is proposed in this work. The bur fraction of horse chestnut was evaluated as a novel and cheap renewable feedstock to obtain valuable compounds suitable for their use in industrial applications. The integrated valorization scheme comprised an initial hydroethanolic extraction of antioxidant compounds (optimized through surface response methodology), the alkaline delignification of the exhausted solid to obtain a lignin-enriched fraction, and the enzymatic digestibility of the remaining cellulose fraction to produce fermentable sugars. In addition, the structural characterization of the extract by FT-IR and TGA was performed, and the analysis by UPLC-DAD-ESI-MS allowed the tentative identification of eleven antioxidant phenolic compounds. The application of this multiproduct valorization approach led to the production of 13 kg antioxidant extracted compounds, 33.2 kg lignin and 14.5 kg glucose per each 100 kg of horse chestnut burs, which demonstrates the great potential of this residue as a biorefinery substrate.
本工作提出了一种从榛果生物废物（城市固体废物）中增值生物活性化合物的生物炼制方案。榛果的外壳部分被评估为一种新颖且廉价的可再生原料，以获得适合工业应用的有价值的化合物。综合增值方案包括抗氧化化合物的初始水醇提取（通过表面响应方法进行优化）、碱性脱木质素以获得富含木质素的部分，以及剩余纤维素部分的酶解以生产可发酵糖。此外，还通过 FT-IR 和 TGA 对提取物进行了结构表征，通过 UPLC-DAD-ESI-MS 分析可以暂定鉴定出 11 种抗氧化酚类化合物。这种多产品增值方法的应用导致每 100 公斤榛果外壳可生产 13 公斤抗氧化提取物化合物、33.2 公斤木质素和 14.5 公斤葡萄糖，这证明了这种废物作为生物炼制底物的巨大潜力。
