Nkambeu Bruno, Salem Jennifer Ben, Beaudry Francis
Groupe de Recherche en Pharmacologie Animal du Québec (GREPAQ), Département de Biomédecine Vétérinaire, Faculté de Médecine Vétérinaire, Université de Montréal, 3200 Sicotte, Saint-Hyacinthe, Québec, J2S 2M2, Canada.
Neurochem Res. 2020 Aug;45(8):1851-1859. doi: 10.1007/s11064-020-03049-4. Epub 2020 May 16.
Capsaicin is the most abundant pungent molecule identified in red chili peppers, and it is widely used for food flavoring, in pepper spray for self-defense devices and recently in ointments for the relief of neuropathic pain. Capsaicin and several other related vanilloid compounds are secondary plant metabolites. Capsaicin is a selective agonist of the transient receptor potential channel, vanilloid subfamily member 1 (TRPV1). After exposition to vanilloid solution, Caenorhabditis elegans wild type (N2) and mutants were placed on petri dishes divided in quadrants for heat stimulation. Thermal avoidance index was used to phenotype each tested C. elegans experimental groups. The data revealed for the first-time that capsaicin can impede nocifensive response of C. elegans to noxious heat (32-35 °C) following a sustained exposition. The effect was reversed 6 h post capsaicin exposition. Additionally, we identified the capsaicin target, the C. elegans transient receptor potential channel OCR-2 and not OSM-9. Further experiments also undoubtedly revealed anti-nociceptive effect for capsaicin analogues, including olvanil, gingerol, shogaol and curcumin.
辣椒素是在红辣椒中发现的最丰富的辛辣分子，它被广泛用于食品调味、自卫设备用的辣椒喷雾中，最近还用于缓解神经性疼痛的药膏中。辣椒素和其他几种相关的香草酸类化合物是植物的次生代谢产物。辣椒素是瞬时受体电位通道香草酸亚家族成员1（TRPV1）的选择性激动剂。将野生型秀丽隐杆线虫（N2）和突变体暴露于香草酸溶液后，放置在划分为象限的培养皿上进行热刺激。使用热回避指数对每个测试的秀丽隐杆线虫实验组进行表型分析。数据首次显示，持续暴露后，辣椒素会阻碍秀丽隐杆线虫对有害热（32-35°C）的伤害性反应。辣椒素暴露6小时后，这种作用会逆转。此外，我们确定了辣椒素的靶点，即秀丽隐杆线虫的瞬时受体电位通道OCR-2，而不是OSM-9。进一步的实验也无疑揭示了辣椒素类似物的抗伤害感受作用，包括奥伐尼、姜辣素、姜烯酚和姜黄素。
