Dyankov Georgi, Eftimov Tinko, Malinowski Nikola, Belina Evdokia, Kisov Hristo, Mikulic Predrag, Bock Wojtek
Institute for Optical Materials and Photonics, Bulgarian Academy of Sciences, 109 Acad. G. Bonchev, Str, Sofia 1113, Bulgaria.
Photonics Research Center, Université du Québec en Outaouais, rue 101 St-Jean Bosco, Gatineau, Québec, J8 X 3G5, Canada.
Data Brief. 2020 Apr 29;30:105641. doi: 10.1016/j.dib.2020.105641. eCollection 2020 Jun.
Matrix-assisted pulsed laser evaporation (MAPLE) is an alternative and complimentary method to pulsed laser deposition. MAPLE has been demonstrated to be a less harmful approach for transporting and depositing delicate, highly sensitive molecules. Metalloproteins are considered sensitive molecules since their bioactivity is determined not only by their chemical structure but also by conformational changes that can be altered by deposition methods. Here we report a dataset of MAPLE deposition parameters of haemoglobin (Hb) that ensures the retention of its bioactivity. Methods for parameters optimization are also described. The data and analysis should be valuable for researchers interested in application of MAPLE techniques for metalloprotein immobilization since it provides a unique opportunity for direct immobilization. The data presents the results of previously conducted experiments on the basis of which is based the research article entitled "A Highly Efficient Biosensor based on MAPLE Deposited Hemoglobin on LPGs Around Phase Matching Turning Point" [1].
基质辅助脉冲激光蒸发（MAPLE）是脉冲激光沉积的一种替代和补充方法。MAPLE已被证明是一种对运输和沉积精细、高敏感分子危害较小的方法。金属蛋白被认为是敏感分子，因为它们的生物活性不仅取决于其化学结构，还取决于可能因沉积方法而改变的构象变化。在此，我们报告了一组血红蛋白（Hb）的MAPLE沉积参数数据集，该数据集可确保其生物活性得以保留。还描述了参数优化方法。这些数据和分析对于对将MAPLE技术应用于金属蛋白固定化感兴趣的研究人员应具有价值，因为它为直接固定提供了独特的机会。这些数据展示了先前进行的实验结果，基于这些结果撰写了题为《基于MAPLE在相位匹配转折点附近的长周期光栅上沉积血红蛋白的高效生物传感器》的研究文章[1]。
