Department of Anesthesiology and Perioperative Medicine, School of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA.
Department of Orthopaedic Surgery, Wakayama Medical University, Wakayama, Japan.
Mol Pain. 2020 Jan-Dec;16:1744806920927852. doi: 10.1177/1744806920927852.
An electrophysiological technique that can record nerve impulses from a single nerve fiber is indispensable for studying modality-specific sensory receptors such as low threshold mechanoreceptors, thermal receptors, and nociceptors. The teased-fiber single-unit recording technique has long been used to resolve impulses that are likely to be from a single nerve fiber. The teased-fiber single-unit recording technique involves tedious nerve separation procedures, causes nerve fiber impairment, and is not a true single-fiber recording method. In the present study, we describe a new and true single-fiber recording technique, the pressure-clamped single-fiber recording method. We have applied this recording technique to mouse whisker hair follicle preparations with attached whisker afferents as well as to skin-nerve preparations made from mouse hindpaw skin and saphenous nerves. This new approach can record impulses from rapidly adapting mechanoreceptors (RA), slowly adapting type 1 mechanoreceptors (SA1), and slowly adapting type 2 mechanoreceptors (SA2) in these tissue preparations. We have also applied the pressure-clamped single-fiber recordings to record impulses on Aβ-fibers, Aδ-fibers, and C-fibers. The pressure-clamped single-fiber recording technique provides a new tool for sensory physiology and pain research.
一种能够从单个神经纤维记录神经冲动的电生理学技术，对于研究特定模态的感觉受体（如低阈值机械感受器、热受体和伤害感受器）是不可或缺的。 teased-fiber 单细胞记录技术长期以来一直用于解析可能来自单个神经纤维的冲动。 teased-fiber 单细胞记录技术涉及繁琐的神经分离程序，会造成神经纤维损伤，并且不是真正的单纤维记录方法。在本研究中，我们描述了一种新的真正的单纤维记录技术，即压力钳制单纤维记录技术。我们已经将这种记录技术应用于带有附接的胡须传入神经的小鼠胡须毛囊准备物以及来自小鼠后足皮肤和隐神经的皮肤-神经准备物。这种新方法可以记录这些组织准备物中的快速适应机械感受器（RA）、缓慢适应型 1 机械感受器（SA1）和缓慢适应型 2 机械感受器（SA2）的冲动。我们还将压力钳制单纤维记录技术应用于记录 Aβ-纤维、Aδ-纤维和 C-纤维的冲动。压力钳制单纤维记录技术为感觉生理学和疼痛研究提供了一种新工具。
