Department of Thermal Science and Energy Engineering, University of Science and Technology of China, Hefei, 230027, China.
School of Life Sciences, University of Science and Technology of China, Hefei, 230027, China.
Analyst. 2020 Jun 15;145(12):4130-4137. doi: 10.1039/d0an00460j.
Antibodies have now been widely used for clinical treatment of a number of tumors. However, there are serious problems associated with antibody therapy, such as potential interactions of antibodies with the immune system as well as long production cycles. Recently, aptamers have been found to function similar to antibodies in terms of affinity and specificity to certain proteins and are attracting much attention for their low immunogenicity, easy chemical synthesis, and efficient penetration into tissues due to their small size. However, how to access high affinity and selectivity aptamers efficiently for further analysis is still open to be resolved. Herein, an aptamer discovery method that combines the continuous flow ddPCR technology with cytometer sorting of beads is reported, such that we have obtained DNA aptamers binding specifically to PD-1 with an affinity of over 60-fold higher than that for the best-reported method.
抗体现已广泛用于多种肿瘤的临床治疗。然而，抗体疗法存在严重问题，例如抗体与免疫系统的潜在相互作用以及生产周期长。最近，发现适体在与某些蛋白质的亲和力和特异性方面与抗体的功能相似，由于其体积小，其免疫原性低、易于化学合成以及高效穿透组织而受到广泛关注。然而，如何有效地获得高亲和力和选择性的适体以进行进一步分析仍有待解决。在此，报道了一种将连续流动 ddPCR 技术与珠细胞分选相结合的适体发现方法，从而获得了与 PD-1 特异性结合的 DNA 适体，其亲和力比报道的最佳方法高 60 多倍。
