Nucleus of Periodontal Research, Dental School, University of Taubaté, Brazil.
Nucleus of Periodontal Research, Dental School, University of Taubaté, Brazil.
Arch Oral Biol. 2020 Aug;116:104747. doi: 10.1016/j.archoralbio.2020.104747. Epub 2020 May 7.
This 6-year study evaluatedAggregatibacter actinomycetemcomitans outcomes and their relationship to clinical status.
From the eligible individuals (23-70 years of age), 31 regular compliers (between-visit interval < 6 months) were randomly selected and matched for age/sex with 31 irregular compliers (between-visit interval > 6 months). Periodontal clinical examination and subgingival samples were obtained 5 times: T1 (baseline), T2 (after active periodontal therapy), T3 (2 years), T4 (4 years), and T5 (6 years). Total bacteria load, A. actinomycetemcomitans, and red complex species Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola levels were determined by qPCR while PCR was used to determine the occurrence of the a-b-c-d-e-f-g serotypes and the JP2 clone of A. actinomycetemcomitans. Data between groups was compared over time.
At baseline PCR revealed A. actinomycetemcomitans prevalence of 9.7 % and JP2 prevalence of 6.7 %. A. actinomycetemcomitans qPCR levels were higher among individuals < 35 years of age and increased at T2 in irregular compliers. At in irregular compliers at the three follow-up visits. Serotypes a, d, and f showed greater values in at least one follow-up visit in regular compliers. A. actinomycetemcomitans showed negative correlation with probing depth (PD) while serotype b showed negative correlations with PD, PI, clinical attachment level and red complex.
Longitudinally, compliance during PMT contributed to lower A. actinomycetemcomitans levels with some degree of correlation with clinical status. However, this study failed to report any positive effect on the occurrence of the most virulent representatives, i.e. serotype b and the JP2 clone.
本为期 6 年的研究评估了伴放线放线杆菌的结果及其与临床状况的关系。
从合格的个体（23-70 岁）中，随机选择 31 名定期依从者（就诊间隔<6 个月），并按年龄/性别与 31 名不定期依从者（就诊间隔>6 个月）进行匹配。在 5 次就诊时进行牙周临床检查和龈下样本采集：T1（基线）、T2（牙周治疗后）、T3（2 年）、T4（4 年）和 T5（6 年）。通过 qPCR 测定总细菌负荷、伴放线放线杆菌和红色复合体物种牙龈卟啉单胞菌、福赛斯坦纳菌、中间普氏菌水平，PCR 用于测定 a-b-c-d-e-f-g 血清型和伴放线放线杆菌 JP2 克隆的发生情况。比较了组间随时间的变化。
基线时，PCR 显示伴放线放线杆菌的患病率为 9.7%，JP2 的患病率为 6.7%。在年龄<35 岁的个体中，伴放线放线杆菌 qPCR 水平较高，不规则依从者在 T2 时增加。在不规则依从者中，在三次随访中均增加。在至少一次随访中，a、d 和 f 型在定期依从者中显示出更高的值。伴放线放线杆菌与探诊深度（PD）呈负相关，而 b 型与 PD、PI、临床附着水平和红色复合体呈负相关。
纵向来看，PMT 期间的依从性导致伴放线放线杆菌水平降低，与一定程度的临床状况相关。然而，本研究未能报告任何对最毒力代表物，即 b 型和 JP2 克隆发生的积极影响。
