Smołucha Grzegorz, Piórkowska Katarzyna, Ropka-Molik Katarzyna, Sikora Jacek
Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland.
Department of Sheep and Goat Breeding, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland.
Animals (Basel). 2020 May 14;10(5):844. doi: 10.3390/ani10050844.
Olkuska is a highly prolific sheep breed in Poland. Thanks to earlier identification of the genetic basis of its prolificacy, a mutation in the gene, we can use molecular biology tools to identify this causative mutation affecting prolificacy. In our research, we used the High-Resolution Melting (HRM) and Sanger sequencing methods to identify the genotypes of the studied animals. The result obtained by the HRM method is identical to those obtained by the sequencing method, which confirms the effectiveness of the HRM method and the possibility of quick and cheap identification of individuals with a FecX mutation.
奥尔库斯卡羊是波兰一种繁殖力很强的绵羊品种。由于早期确定了其繁殖力的遗传基础，即该基因中的一种突变，我们可以使用分子生物学工具来鉴定这种影响繁殖力的致病突变。在我们的研究中，我们使用高分辨率熔解曲线分析（HRM）和桑格测序方法来鉴定所研究动物的基因型。HRM方法获得的结果与测序方法获得的结果相同，这证实了HRM方法的有效性以及快速廉价鉴定携带FecX突变个体的可能性。
