Templeman T S, Stein D B, DeMaggio A E
Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755.
Biochem Genet. 1988 Oct;26(9-10):595-603. doi: 10.1007/BF02399604.
The ostrich fern, Matteuccia struthiopteris L., contains two globulin spore storage proteins of 2.2 S and 11.3 S, with physical characteristics similar to those of seed storage proteins of Brassica napus (rapeseed) and Raphanus sativus (radish). By the use of a cloned cDNA that encodes the 1.7 S B. napus storage protein (napin), gene sequences that hybridized with napin were detected in fern nuclear DNA, and a 900-nucleotide homologous mRNA was detected in developing spores. In vitro translation of this fern mRNA produced a 22-kD polypeptide comparable in size to the 21-kD precursor polypeptide identified in Brassica. No hybridizations were observed between the Brassica 12 S clone and either fern DNA or developing spore mRNA.
鸵鸟蕨(Matteuccia struthiopteris L.)含有两种球蛋白孢子储存蛋白,分别为2.2 S和11.3 S,其物理特性与甘蓝型油菜(油菜籽)和萝卜(萝卜)的种子储存蛋白相似。通过使用编码1.7 S甘蓝型油菜储存蛋白(napin)的克隆cDNA,在蕨类植物核DNA中检测到与napin杂交的基因序列,并在发育中的孢子中检测到900个核苷酸的同源mRNA。这种蕨类植物mRNA的体外翻译产生了一种22-kD的多肽,其大小与在甘蓝型油菜中鉴定出的21-kD前体多肽相当。在甘蓝型油菜12 S克隆与蕨类植物DNA或发育中的孢子mRNA之间未观察到杂交现象。