Hari Khetan Multiple Campus, Birgunj, Nepal (affiliated to Tribhuvan University, Kirtipur, Nepal).
B and B Medical Institute, Lalitpur, Nepal.
Mol Cell Probes. 2020 Oct;53:101599. doi: 10.1016/j.mcp.2020.101599. Epub 2020 May 18.
•Most of the COVID-19 cases in Nepal are in the Southern districts of Nepal bordering India with travel histories to India.•Very few positive cases of COVID-19 are detected in Nepal which could either be due to early national lockdown.•Low PCR positivity rates could also be due to inefficiency of the PCR methods.•Whole genomes of 93 clinical samples from COVID-19 patients were analyzed to find the primer and probe binding sites.•Mutations in probe binding sites were found which could impact PCR efficiency resulting in false negative results.
•尼泊尔大多数 COVID-19 病例都在与印度接壤的尼泊尔南部地区,这些地区的人有前往印度的旅行史。•尼泊尔发现的 COVID-19 阳性病例很少,这可能是由于全国早期封锁。•PCR 阳性率低也可能是由于 PCR 方法效率低下。•对 93 例来自 COVID-19 患者的临床样本的全基因组进行了分析,以找到引物和探针结合位点。•在探针结合位点发现了突变,这可能会影响 PCR 效率,导致假阴性结果。