Igura Saori, Nagatani Mariko, Kasahara Kenichiro, Andoh Rie, Fukunaga Yachiyo, Hashiguchi Osamu, Okazaki Shuzo, Yamaguchi Yuko
Gotemba Laboratory, BoZo Research Center Inc., 1284 Kamado, Gotemba, Shizuoka 412-0039, Japan.
Tsukuba Research Institute, BoZo Research Center Inc., 8 Okubo, Tsukuba, Ibaraki 300-2611, Japan.
J Toxicol Pathol. 2020 Apr;33(2):115-119. doi: 10.1293/tox.2019-0079. Epub 2020 Jan 25.
To examine the biological and morphological features of tubular aggregates (TAs) in the skeletal muscles of non-obese diabetic/Cg /ShiJic (NOG) mice, 73 male and 72 female specific-pathogen-free NOG mice were examined at 7, 18, 22, 26, and 52 weeks of age. TAs were observed as intracytoplasmic eosinophilic materials of the femoral muscles in males at 18, 22, 26, and 52 weeks of age and in females at 52 weeks of age; gender-related differences were noted in the onset time and lesion degree. Intracytoplasmic materials were positive for Gomori's trichrome stain. Electron microscopy revealed that TAs were composed of an accumulation of dilated sarcoplasmic reticulum. In addition, TAs were observed in the femoral and gastrocnemius muscles, but not in the soleus and diaphragm muscles, suggesting that TAs are present in fast muscle fibers. The morphology of TAs and the type of myofibers involved, as well as the gender difference in NOG mice were essentially the same as those of TAs observed in C57BL/6J and MRL+/+ mice.
为研究非肥胖糖尿病/ Cg / ShiJic（NOG）小鼠骨骼肌中管状聚集体（TA）的生物学和形态学特征，对73只雄性和72只雌性无特定病原体的NOG小鼠在7、18、22、26和52周龄时进行了检查。在18、22、26和52周龄的雄性以及52周龄的雌性小鼠的股四头肌中，TA被观察为胞质内嗜酸性物质；在发病时间和病变程度上存在性别差异。胞质内物质对Gomori三色染色呈阳性。电子显微镜显示TA由扩张的肌浆网积聚组成。此外，在股四头肌和腓肠肌中观察到TA，但在比目鱼肌和膈肌中未观察到，这表明TA存在于快肌纤维中。NOG小鼠中TA的形态、所涉及的肌纤维类型以及性别差异与在C57BL / 6J和MRL + / +小鼠中观察到的TA基本相同。
