利比亚的黎波里后院鸡群中新城疫病毒的血清学和分子检测。
Seroprevalence and molecular detection of Newcastle disease virus in backyard chickens in Tripoli, Libya.
机构信息
Department of Microbiology, Faculty of Veterinary Sciences, University of Aljfara, Aljfara, Libya.
Contributed equally in this study.
出版信息
Open Vet J. 2020 Apr;10(1):80-85. doi: 10.4314/ovj.v10i1.13. Epub 2020 Mar 20.
BACKGROUND
Newcastle disease (ND) is a viral disease that affecting many avian species all over the world.
AIM
ND has been successfully controlled by the vaccination of commercial poultry in Libya. However, there was a lack of information about the situation of ND in backyard chickens. Therefore, this study determined the prevalence of ND in backyard chickens in different locations of Tripoli.
METHODS
A total number of 280 cloacal swabs (190 in summer and 90 in winter) and 412 sera were collected from non-vaccinated backyard chicken flocks in different geographical locations within the area of Tripoli namely Qasr Ben Ghashier, Al-Sawani, Souq Al-Gomaa, Tajourah, Ein Zara, and Janzour. Cloacal swabs and sera were tested by real time polymerase chain reaction (PCR) and ELISA, respectively.
RESULTS
The prevalence of ND virus (NDV) infection in backyard chickens in different locations of Tripoli during summer and winter was 45% using real-time reverse transcription-PCR. Except in Qasr Ben Ghashier, the prevalence in summer season was significantly higher than in winter ( = 46.13, ≥ 0.00001). ELISA test revealed 218 positive out of 412 tested samples with total prevalence of 53% across the city of Tripoli in all regions. Obviously, Qasr Ben Ghashier had significantly ( = 74.09, ≥ 0.00001) the highest prevalence (82%) of NDV specific antibodies followed by Tajourah (68%).
CONCLUSION
This study demonstrated the situation of ND in backyard chicken highlighting the necessity of a comprehensive vaccination plan for backyard chickens.
背景
新城疫（ND）是一种影响世界范围内许多禽类物种的病毒性疾病。
目的
在利比亚，通过对商业家禽进行疫苗接种，ND 已得到成功控制。然而，关于后院鸡中 ND 情况的信息却很缺乏。因此，本研究旨在确定不同地点的后院鸡中 ND 的流行情况。
方法
从的黎波里不同地理位置的非接种后院鸡群中采集了总共 280 份直肠拭子（夏季 190 份，冬季 90 份）和 412 份血清。使用实时聚合酶链反应（PCR）和酶联免疫吸附试验（ELISA）分别对直肠拭子和血清进行了检测。
结果
在夏季和冬季，使用实时逆转录-PCR 检测到来自的黎波里不同地点后院鸡中 ND 病毒（NDV）感染的流行率分别为 45%。除了 Qasr Ben Ghashier 外，夏季的流行率明显高于冬季（ = 46.13， ≥ 0.00001）。ELISA 检测显示，在 412 份测试样本中，有 218 份呈阳性，整个的黎波里市各地区的总流行率为 53%。显然，Qasr Ben Ghashier 具有最高的 NDV 特异性抗体流行率（82%），明显高于其他地区（ = 74.09， ≥ 0.00001），其次是 Tajourah（68%）。
结论
本研究表明了后院鸡中 ND 的情况，强调了对后院鸡进行全面疫苗接种计划的必要性。
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