Pisanu Salvatore, Cacciotto Carla, Pagnozzi Daniela, Uzzau Sergio, Pollera Claudia, Penati Martina, Bronzo Valerio, Addis Maria Filippa
Porto Conte Ricerche, Alghero, Italy.
Dipartimento di Scienze Biomediche, Università degli Studi di Sassari, Sassari, Italy.
Data Brief. 2020 May 6;30:105665. doi: 10.1016/j.dib.2020.105665. eCollection 2020 Jun.
We present a proteomic dataset generated from half-udder Alpine goat milk. The milk samples belonged to 3 groups: i) mid-lactation, low somatic cell count, uninfected milk (MLU, n=3); ii) late lactation, high somatic cell count, uninfected milk (LHU, n=3); and late lactation, high somatic cell count, subclinically infected milk (LHS, n=3). The detailed description of results is reported in the research article entitled "Impact of infection on the late lactation goat milk proteome: new perspectives for monitoring and understanding mastitis in dairy goats". After milk defatting, high speed centrifugation and trypsin digestion of milk with the FASP protocol, peptide mixtures were analyzed by LC-MS/MS on a Q-Exactive. Peptide identification was carried out using Sequest-HT in Proteome Discoverer. Then, the Normalized Abundance Spectrum Factor (NSAF) value was calculated by label free quantitation using the spectral counting approach, and Gene Ontology (GO) annotation by Uniprot was carried out by reporting biological process, molecular function and cellular component. The MS data have been deposited to the ProteomeXchange via the PRIDE with the dataset identifier PXD017243.
我们展示了一个源自阿尔卑斯山羊半份乳房乳汁的蛋白质组数据集。乳汁样本分为3组：i）泌乳中期、低体细胞计数、未感染的乳汁（MLU，n = 3）；ii）泌乳后期、高体细胞计数、未感染的乳汁（LHU，n = 3）；以及泌乳后期、高体细胞计数、亚临床感染的乳汁（LHS，n = 3）。结果的详细描述发表在题为《感染对泌乳后期山羊乳蛋白质组的影响：监测和理解奶山羊乳腺炎的新视角》的研究文章中。对乳汁进行脱脂、高速离心并用FASP方案进行胰蛋白酶消化后，肽混合物在Q-Exactive上通过LC-MS/MS进行分析。使用Proteome Discoverer中的Sequest-HT进行肽段鉴定。然后，采用光谱计数法通过无标记定量计算标准化丰度谱因子（NSAF）值，并通过Uniprot进行基因本体（GO）注释，报告生物学过程、分子功能和细胞成分。质谱数据已通过PRIDE存入ProteomeXchange，数据集标识符为PXD017243。
