从粗甘油中生产聚羟基烷酸酯的培养物富集的底物策略优化。
Substrate strategy optimization for polyhydroxyalkanoates producing culture enrichment from crude glycerol.
机构信息
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
出版信息
Bioresour Technol. 2020 Sep;311:123516. doi: 10.1016/j.biortech.2020.123516. Epub 2020 May 11.
Crude glycerol is by-product produced from biodiesel industry and can be converted directly by mixed microbial culture (MMC) into polyhydroxyalkanoates (PHAs). This study investigated the effects of the reverse (SBR_A) and positive (SBR_B) glycerol gradient substrate strategy on PHA-accumulating culture enrichment and the maximum PHA accumulating stability under substrates with different glycerol and volatile fatty acid (VFA) proportion. The results showed that crude glycerol was mainly used for PHA production rather than biomass growth in SBR_A. The maximum q was 0.65 g COD/g X·h under sole crude glycerol condition in SBR_A, which was 2.41 times higher than that of SBR_B. Moreover, the PHA accumulating ability of the biomass from SBR_A was more stable than SBR_B. Saccharibacteria_genera_incertae_sedis was for the first time found to be the dominant genus using crude glycerol for PHA production. This research provides an insight into enrichment strategy to effectively enrich PHA-accumulating culture from crude glycerol.
粗甘油是生物柴油工业的副产品，可以直接通过混合微生物培养（MMC）转化为聚羟基烷酸酯（PHA）。本研究考察了反向（SBR_A）和正向（SBR_B）甘油浓度底物策略对 PHA 积累培养物富集的影响，以及在不同甘油和挥发性脂肪酸（VFA）比例的底物下最大 PHA 积累稳定性。结果表明，在 SBR_A 中，粗甘油主要用于 PHA 生产而不是生物量生长。在 SBR_A 中仅使用粗甘油的条件下，最大 q 值为 0.65 g COD/g X·h，比 SBR_B 高 2.41 倍。此外，来自 SBR_A 的生物质的 PHA 积累能力比 SBR_B 更稳定。首次发现 Saccharibacteria_genera_incertae_sedis 是利用粗甘油生产 PHA 的优势属。本研究为从粗甘油中有效富集 PHA 积累培养物的富集策略提供了深入的了解。
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