Department of Molecular Medicine and Pathology, School of Medical Sciences and Maurice Wilkins Centre for Biomolecular Discovery, The University of Auckland, Auckland, New Zealand.
Methods Mol Biol. 2020;2136:271-278. doi: 10.1007/978-1-0716-0467-0_20.
The critical first step of Group A Streptococcus (GAS) pathogenesis is adhesion to the host pharyngeal and skin epithelial cell surfaces (Brouwer et al., FEBS Lett 590:3739-3757, 2016). Host-cell adhesion assays provide a straightforward model to study these host-pathogen interactions. Here, we describe the culturing of immortalized cell lines into monolayers to mimic host epithelia. Various GAS strains can then be added to study their adhesion properties. In addition, we describe the use of antibodies raised against the cell-surface components of GAS to study if these are able to neutralize the binding of GAS to the cell lines. This provides an indication if these cell-surface components are involved in adhesion and if antibodies generated against them function through neutralization.
A 组链球菌（GAS）发病机制的关键第一步是黏附于宿主咽和皮肤上皮细胞表面（Brouwer 等人，FEBS Lett 590:3739-3757, 2016）。宿主细胞黏附测定为研究这些宿主-病原体相互作用提供了一种直接的模型。在这里，我们描述了将永生化细胞系培养成单层以模拟宿主上皮细胞的方法。然后可以添加各种 GAS 菌株来研究它们的黏附特性。此外，我们还描述了使用针对 GAS 细胞表面成分的抗体来研究这些抗体是否能够中和 GAS 与细胞系的结合。这表明这些细胞表面成分是否参与黏附，以及针对它们产生的抗体是否通过中和起作用。
