Shenyang Pharmaceutical University, Shenyang, 110016, China.
Institutes of Biomedical Sciences, Shanxi University, Taiyuan, 030006, China.
Future Microbiol. 2020 Jun;15:713-721. doi: 10.2217/fmb-2020-0026. Epub 2020 May 20.
To characterize two plasmids p13294-KPC and pA1966-NR from clinical strains. Plasmids p13294-KPC and pA1966-NR were fully sequenced and then detailed genomic analysis was performed in this work. The antimicrobial resistance phenotypes were determined. p13294-KPC and pA1966-NR displayed Inc:IncFII dual-replicon structures. The backbone of these two plasmids were closely related to each other. p13294-KPC contained two accessory modules, namely ΔIS and region, and the region carried a range of mobile elements and resistance gene . while pA1966-NR contained four individual IS elements in its backbone and carried no resistance genes. This study provided a deeper insight into the genomic characterization of Inc: IncFII type plasmids p13294-KPC and pA1966-NR.
对临床分离株中的 2 个质粒 p13294-KPC 和 pA1966-NR 进行特征分析。 对质粒 p13294-KPC 和 pA1966-NR 进行了全序列测序，并在本工作中进行了详细的基因组分析。 测定了这些质粒的抗微生物表型。 p13294-KPC 和 pA1966-NR 显示 Inc:IncFII 双复制子结构。 这两个质粒的骨架彼此密切相关。 p13294-KPC 包含两个辅助模块，即 ΔIS 和 区域，而 区域携带一系列移动元件和耐药基因 。 而 pA1966-NR 则在其骨架中包含 4 个单独的 IS 元件，不携带耐药基因。 本研究深入了解了 Inc:IncFII 型质粒 p13294-KPC 和 pA1966-NR 的基因组特征。
