Liu Si Yuan, Yi Guo Qiang, Tang Zhong Lin, Chen Bin
College of Animal Science & Technology, Hunan Agricultural University, Changsha 410128, China.
Genome Analysis Laboratory of the Ministry of Agriculture, Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China.
Yi Chuan. 2020 May 20;42(5):435-443. doi: 10.16288/j.yczz.19-390.
The CRISPR/Cas9 system is a powerful tool which has been extensively used for genome editing in the past few years. Nuclease-dead Cas9 (CRISPR/dCas9), a Cas9 protein mutant without splicing ability, along with loss-of- function (LOF), gain-of-function (GOF), or non-coding genes scanning approaches can reveal genome-scale functional determinants. CRISPR/Cas9 has been widely adopted to decipher disease mechanisms and pinpoint drug targets in the life science field, and also provide novel insights into animal genetics and breeding. In this review, we summarize the research progress in high-throughput CRISPR/Cas9 screening for revealing the functional genes and regulatory elements in the whole genome. We also highlight the applications of CRISPR/Cas9 system in the animal cells, providing a reference for gene editing and other related research in related fields.
CRISPR/Cas9系统是一种强大的工具，在过去几年中已被广泛用于基因组编辑。核酸酶失活的Cas9（CRISPR/dCas9）是一种没有剪接能力的Cas9蛋白突变体，与功能丧失（LOF）、功能获得（GOF）或非编码基因扫描方法一起，可以揭示全基因组范围内的功能决定因素。CRISPR/Cas9已被广泛用于破译生命科学领域的疾病机制和确定药物靶点，也为动物遗传学和育种提供了新的见解。在这篇综述中，我们总结了高通量CRISPR/Cas9筛选在揭示全基因组功能基因和调控元件方面的研究进展。我们还强调了CRISPR/Cas9系统在动物细胞中的应用，为相关领域的基因编辑及其他相关研究提供参考。
