Hu Ya Li, Dai Rui, Liu Yong Xin, Zhang Jing Ying, Hu Bin, Chu Cheng Cai, Yuan Huai Bo, Bai Yang
School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China.
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Yi Chuan. 2020 May 20;42(5):506-518. doi: 10.16288/j.yczz.20-070.
The root-associated bacterial microbiota is closely related to life activities of land plants, and its composition is affected by geographic locations and plant genotypes. However, the influence of plant genotypes on root microbiota in rice grown in northern China remains to be explained. In this study, we performed 16S rRNA gene amplicon sequencing to generate bacterial community profiles of two representative rice cultivars, Nipponbare and IR24. They are planted in Changping and Shangzhuang farms in Beijing and have reached the reproductive stage. We compared their root microbiota in details by Random Forest machine learning algorithm and network analysis. We found that the diversity of rice root microbiota was significantly affected by geographic locations and rice genotypes. Nipponbare and IR24 showed distinct taxonomic composition of the root microbiota and the interactions between different bacteria. Moreover, the root bacteria could be used as biomarkers to distinguish Nipponbare from IR24 across regions. Our study provides a theoretical basis for the in-depth understanding of rice root microbiota in Northern China and the improvement of rice breeding from the perspective of the interaction between root microorganisms and plants.
根际相关细菌微生物群与陆地植物的生命活动密切相关，其组成受地理位置和植物基因型的影响。然而，植物基因型对中国北方种植水稻根际微生物群的影响仍有待阐明。在本研究中，我们进行了16S rRNA基因扩增子测序，以生成两个代表性水稻品种日本晴和IR24的细菌群落图谱。它们种植在北京昌平农场和上庄农场，已进入生殖阶段。我们通过随机森林机器学习算法和网络分析详细比较了它们的根际微生物群。我们发现，水稻根际微生物群的多样性受地理位置和水稻基因型的显著影响。日本晴和IR24的根际微生物群在分类组成和不同细菌之间的相互作用方面表现出明显差异。此外，根际细菌可作为跨区域区分日本晴和IR24的生物标志物。我们的研究为深入了解中国北方水稻根际微生物群以及从根际微生物与植物相互作用的角度改良水稻育种提供了理论依据。
