通过聚丙烯酰胺梯度凝胶电泳法测定天然蛋白质的分子量
Determination of molecular weight of native proteins by polyacrylamide gradient gel electrophoresis.
作者信息
Nishizawa H, Kita N, Okimura S, Takao E, Abe Y
机构信息
Kyoritsu College of Pharmacy, Tokyo, Japan.
出版信息
Electrophoresis. 1988 Dec;9(12):803-6. doi: 10.1002/elps.1150091203.
PMID:3243233
Abstract
An improved method for the estimation of molecular weights of native proteins by polyacrylamide gel electrophoresis, in 9 cm x 9 cm x 0.05 mm 4-20% T fabric reinforced gradient gels, is described. Plotting the logarithm of the relative mobilities of proteins versus gel concentrations produces lines whose slopes are related to molecular weights.
摘要
本文描述了一种通过聚丙烯酰胺凝胶电泳在9厘米×9厘米×0.05毫米的4-20%T织物增强梯度凝胶中估算天然蛋白质分子量的改进方法。绘制蛋白质相对迁移率的对数与凝胶浓度的关系图会产生斜率与分子量相关的直线。
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