Abbal M, Julie C
Service d'immunologie CHU, Rangueil, Tolouse, France.
Electrophoresis. 1988 Dec;9(12):844-5. doi: 10.1002/elps.1150091211.
Electrophoretic transfer of protein after isolectric focusing using a polyacrylamide gel of less than 0.5 mm is difficult if the gel is backed to an electrically nonconductive casting support, such as glass plate or plastic films. By casting the gel on a cellophane sheet, it is not necessary to remove the gel from the support prior to electrophoretic transfer. The use of a cellophane support does not alter the quality of the final pattern.
如果在小于0.5毫米的聚丙烯酰胺凝胶上进行等电聚焦后进行蛋白质的电泳转移，而凝胶又被支撑在不导电的铸型支持物上，如玻璃板或塑料薄膜，那么转移就会很困难。通过将凝胶浇铸在玻璃纸上，在进行电泳转移之前就无需从支持物上取下凝胶。使用玻璃纸支持物不会改变最终图谱的质量。
