Department of Gynaecology, Xianyang Central Hospital, Xianyang, China.
Eur Rev Med Pharmacol Sci. 2020 May;24(9):4687-4696. doi: 10.26355/eurrev_202005_21156.
In cervical carcinoma (CC), microRNAs (miRNAs) were reported to be involved in its development. In this study, we explored how miR-377-3p regulates cell metastasis and epithelial-mesenchymal transition (EMT) in CC.
Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR), Dual-Luciferase Assay, transwell assays, and Western blot analysis were performed to explore the dysregulation of miR-377-3p.
MiR-377-3p expression was decreased in CC, and the downregulation of miR-377-3p could predict poor prognosis in CC patients. Moreover, miR-377-3p overexpression repressed cell invasion and migration in CC. Similarly, miR-377-3p overexpression also inhibited EMT in CC cells. Furthermore, miR-377-3p directly targeted SGK3 in CC cells. SGK3 silence had the same function as miR-377-3p overexpression in CC. Especially, the upregulation of SGK3 abolished the inhibitory action of miR-377-3p in CC.
Taken together, miR-377-3p inhibited cell metastasis and EMT by suppressing SGK3 expression. Moreover, the high miR-377-3p expression could predict good prognosis of CC patients.
在宫颈癌（CC）中，已有研究报道 microRNAs（miRNAs）参与其发生发展。本研究旨在探讨 miR-377-3p 如何调控 CC 细胞转移和上皮间质转化（EMT）。
采用实时定量聚合酶链反应（qRT-PCR）、双荧光素酶报告基因检测、Transwell 实验和 Western blot 分析来探究 miR-377-3p 的失调情况。
miR-377-3p 在 CC 中呈下调表达，且 miR-377-3p 的下调可预测 CC 患者的不良预后。此外，miR-377-3p 的过表达可抑制 CC 细胞的侵袭和迁移。同样，miR-377-3p 的过表达也可抑制 CC 细胞的 EMT。进一步研究发现，miR-377-3p 可直接靶向 CC 细胞中的 SGK3。CC 细胞中沉默 SGK3 可发挥与 miR-377-3p 过表达相同的作用。特别是，SGK3 的上调可消除 miR-377-3p 在 CC 中的抑制作用。
综上所述，miR-377-3p 通过抑制 SGK3 的表达抑制细胞转移和 EMT。此外，miR-377-3p 的高表达可预测 CC 患者的良好预后。
