Department of Urology, Drum Tower Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, P.R. China.
Eur Rev Med Pharmacol Sci. 2020 May;24(9):4719-4728. doi: 10.26355/eurrev_202005_21160.
Long non-coding RNA (lncRNA) is closely associated with cancer occurrence and tumor development. However, the biological function of lncRNA ZNFX1-AS1 has not yet been reported in bladder cancer. The present study aimed to study the function of ZNFX1-AS1 in bladder cancer cells and the mechanism involved.
The expression of ZNFX1-AS1 in bladder cancer tumor tissues and cell lines was examined by qRT-PCR. The effects of ZNFX1-AS1 knockdown on cell proliferation, cell cycle, cell migration, and invasion were assessed by Cell Counting Kit-8, flow cytometry (FCM), and transwell assays. Bioinformatics analyses and Luciferase reporter assays were performed to explore the mechanism by which ZNFX1-AS1 exerted its oncogenesis role in bladder cancer. The anti-tumor effect of ZNFX1-AS1 silencing on bladder cancer in vivo was also evaluated.
ZNFX1-AS1 was over-expressed in bladder cancer tumor tissues and cell lines. ZNFX1-AS1 expression was found to be associated with tumor size and advanced clinical stage in patients with bladder cancer. Downregulation of ZNFX1-AS1 inhibited cell proliferation, cell clone formation, migration, and invasion of bladder cancer cells. ZNFX1-AS1 was found to interact with miR-193a-3p/Syndecan 1 (SDC1). ZNFX1-AS1 expression was negatively correlated with miR-193a-3p expression, but positively correlated with SDC1 expression in bladder cancer samples. ZNFX1-AS1 knockdown also effectively suppressed tumor growth in an in vivo xenograft model.
ZNFX1-AS1 regulated bladder cancer progression by targeting the miR-193a-3p/SDC1 axis. Our study may provide novel insights for bladder cancer prognosis and therapy.
长链非编码 RNA（lncRNA）与癌症的发生和肿瘤的发展密切相关。然而，lncRNA ZNFX1-AS1 在膀胱癌中的生物学功能尚未报道。本研究旨在研究 ZNFX1-AS1 在膀胱癌细胞中的功能及其涉及的机制。
通过 qRT-PCR 检测膀胱癌肿瘤组织和细胞系中 ZNFX1-AS1 的表达。通过细胞计数试剂盒-8（Cell Counting Kit-8）、流式细胞术（Flow cytometry，FCM）和 Transwell 测定评估 ZNFX1-AS1 敲低对细胞增殖、细胞周期、细胞迁移和侵袭的影响。通过生物信息学分析和荧光素酶报告基因实验探讨 ZNFX1-AS1 在膀胱癌中发挥致癌作用的机制。还评估了 ZNFX1-AS1 沉默对膀胱癌体内抗肿瘤作用。
ZNFX1-AS1 在膀胱癌肿瘤组织和细胞系中过表达。在膀胱癌患者中，ZNFX1-AS1 的表达与肿瘤大小和临床晚期有关。下调 ZNFX1-AS1 抑制膀胱癌细胞的增殖、细胞克隆形成、迁移和侵袭。发现 ZNFX1-AS1 与 miR-193a-3p/ Syndecan 1（SDC1）相互作用。ZNFX1-AS1 的表达与膀胱癌样本中 miR-193a-3p 的表达呈负相关，与 SDC1 的表达呈正相关。在体内异种移植模型中，ZNFX1-AS1 敲低也能有效抑制肿瘤生长。
ZNFX1-AS1 通过靶向 miR-193a-3p/SDC1 轴调控膀胱癌的进展。我们的研究可能为膀胱癌的预后和治疗提供新的思路。
