长非编码 RNA linc01433 通过海绵吸附 miR-1301 促进食管鳞状细胞癌的发生发展。
Long non-coding RNA linc01433 promotes tumorigenesis and progression in esophageal squamous cell carcinoma by sponging miR-1301.
机构信息
Department of Thoracic Surgery, The Third Affiliated Hospital of Soochow University, Changzhou, China.
出版信息
Eur Rev Med Pharmacol Sci. 2020 May;24(9):4785-4792. doi: 10.26355/eurrev_202005_21167.
OBJECTIVE
Long non-coding RNAs (lncRNAs) have emerged as pivotal participants of various tumors. This manuscript focuses on the function of lncRNA linc01433 (linc01433) in esophageal squamous cell carcinoma (ESCC) development.
PATIENTS AND METHODS
Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was performed to detect expressions of linc01433 and microRNA-1301 (miR-1301) in ESCC tissues and cells. Cell counting kit-8 (CCK-8) and colony formation assays were used to verify proliferative ability changes in ESCC influenced by linc01433 and miR-1303. Wound healing and transwell assays were chosen to determine migratory ability in ESCC cells.
RESULTS
Linc01433 was abnormally up-regulated in ESCC tissues and cells. High level of linc01433 was positively correlated with tumor size and lymph node metastasis in ESCC patients. Up-regulation of linc01433 promoted cell proliferation and migration. MiR-1301 was a potential target of linc01433, and its level was negatively regulated by linc01433. MiR-1301 was responsible for linc01433-regulated proliferation and migration of ESCC.
CONCLUSIONS
Linc01433 participated in ESCC progression by regulating miR-1301 and it could function as a novel biomarker in ESCC diagnosis and treatment.
目的
长链非编码 RNA（lncRNA）已成为多种肿瘤的关键参与者。本文重点研究 lncRNA linc01433（linc01433）在食管鳞状细胞癌（ESCC）发展中的作用。
患者和方法
采用实时定量聚合酶链反应（qRT-PCR）检测 ESCC 组织和细胞中 linc01433 和 microRNA-1301（miR-1301）的表达。细胞计数试剂盒-8（CCK-8）和集落形成实验用于验证 linc01433 和 miR-1303 对 ESCC 增殖能力的影响。划痕愈合和 Transwell 实验用于确定 ESCC 细胞的迁移能力。
结果
linc01433 在 ESCC 组织和细胞中异常上调。高水平的 linc01433 与 ESCC 患者的肿瘤大小和淋巴结转移呈正相关。上调 linc01433 促进了细胞的增殖和迁移。miR-1301 是 linc01433 的潜在靶标，其水平受 linc01433 负调控。miR-1301 负责 linc01433 调节的 ESCC 增殖和迁移。
结论
linc01433 通过调节 miR-1301 参与 ESCC 进展，可作为 ESCC 诊断和治疗的新型生物标志物。
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