Yuan R-X, Bao D, Zhang Y
Department of Ultrasound, Shanxian Central Hospital, Heze, China.
Eur Rev Med Pharmacol Sci. 2020 May;24(9):4863-4872. doi: 10.26355/eurrev_202005_21175.
Breast cancer (BC) is the most common malignant tumor in women. We aimed at investigating the function of long non-coding RNA LINC00707 in BC and the potential mechanism.
The expression level of linc00707 was determined using the quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) in BC tissues and cell lines. The Cell Counting Kit-8 (CCK-8) and colony formation assays were performed to detect the potential influence of LINC0070 on the proliferation ability of the BC cells. Also, the invasion and migration abilities were assessed by the transwell assay. Furthermore, with the bioinformatic analysis and the Dual-Luciferase Reporter Gene Assay, we analyzed the interaction in LINC00707/miR-30c/CTHRC1 regulatory loop. The regulatory effects of LINC00707/miR-30c/CTHRC1 on BC were finally determined.
LINC00707 was significantly upregulated in BC tissues and cell lines. The knockdown of LINC00707 inhibited proliferation, invasion, and migration in MDA-MB-231 cells, while the overexpression of LINC00707 achieved the opposite results in MDA-MB-468 cells. LINC00707, acting as a competing endogenous RNA (ceRNA), could sponge miR-30c to upregulate CTHRC1, thus promoting BC progression.
LINC00707 was highly expressed in BC tissues and cells. It promoted cell proliferation, invasion, and migration via miR-30c/CTHRC1 regulatory loop. This might provide a novel target for the diagnosis, treatment, and prognosis for BC.
乳腺癌（BC）是女性最常见的恶性肿瘤。我们旨在研究长链非编码RNA LINC00707在BC中的功能及潜在机制。
采用定量实时聚合酶链反应（qRT-PCR）检测BC组织和细胞系中linc00707的表达水平。进行细胞计数试剂盒-8（CCK-8）和集落形成试验，以检测LINC0070对BC细胞增殖能力的潜在影响。此外，通过transwell试验评估侵袭和迁移能力。此外，通过生物信息学分析和双荧光素酶报告基因试验，我们分析了LINC00707/miR-30c/CTHRC1调控环中的相互作用。最终确定了LINC00707/miR-30c/CTHRC1对BC的调控作用。
LINC00707在BC组织和细胞系中显著上调。敲低LINC00707可抑制MDA-MB-231细胞的增殖、侵袭和迁移，而在MDA-MB-468细胞中过表达LINC00707则得到相反的结果。LINC00707作为一种竞争性内源性RNA（ceRNA），可以吸附miR-30c从而上调CTHRC1表达，进而促进BC进展。
LINC00707在BC组织和细胞中高表达。它通过miR-30c/CTHRC1调控环促进细胞增殖、侵袭和迁移。这可能为BC诊断、治疗及预后提供新靶点。
