小鼠mdx基因座的分子与遗传图谱

Molecular and genetic mapping of the mouse mdx locus.

作者信息

Cavanna J S, Coulton G, Morgan J E, Brockdorff N, Forrest S M, Davies K E, Brown S D

机构信息

Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, London, United Kingdom.

出版信息

Genomics. 1988 Nov;3(4):337-41. doi: 10.1016/0888-7543(88)90124-3.

DOI:10.1016/0888-7543(88)90124-3

PMID:3243547

Abstract

mdx is an X-linked muscular dystrophy mutant of the mouse and a putative homolog of the human X-linked muscular dystrophy locus--Duchenne muscular dystrophy (DMD). Utilizing a C57BL/10/Mus Spretus interspecific cross in which the mdx mutation was segregating, we have constructed a detailed genetic map around the mdx locus on the mouse X chromosome. We were unable to detect recombinants between mdx and exonic probes derived from the human DMD gene. These genetic data support the contention from biochemical studies (E.P. Hoffman, R. H. Brown, and L. M. Kunkel, 1987, Cell 51: 919-928) that DMD and mdx are homologous genes.

摘要

mdx是小鼠的一种X连锁型肌营养不良突变体，也是人类X连锁型肌营养不良基因座——杜兴氏肌营养不良（DMD）的一个假定同源物。利用C57BL/10/Mus Spretus种间杂交（其中mdx突变正在分离），我们构建了围绕小鼠X染色体上mdx基因座的详细遗传图谱。我们无法检测到mdx与源自人类DMD基因的外显子探针之间的重组体。这些遗传数据支持了生化研究（E.P.霍夫曼、R.H.布朗和L.M.孔克尔，1987年，《细胞》51卷：919 - 928页）得出的DMD和mdx是同源基因的观点。