Gómez Evangelina, Cassani María Florencia, Lucero María Soledad, Parreño Viviana, Chimeno Zoth Silvina, Berinstein Analía
Instituto de Agrobiotecnología y Biología Molecular (IABIMO), Instituto Nacional de Tecnología Agropecuaria (INTA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), De los Reseros y Nicolás Repetto s/n, 1686, Hurlingham, Buenos Aires, Argentina.
Universidad Nacional de Luján, Ruta 5 y Avenida Constitución, 6700, Luján, Buenos Aires, Argentina.
AMB Express. 2020 May 20;10(1):95. doi: 10.1186/s13568-020-01029-z.
Infectious bursal disease virus (IBDV) is the etiological agent of an immunosuppressive and highly contagious disease that affects young birds, thus causing important economic losses in the poultry industry. Multimeric particles with different architectures based on the capsid protein VP2 have been widely produced for different purposes. We hereby show the production and easy recovery of IBDV subviral particles (SVP) from transiently transformed Nicotiana benthamiana. The SVP, which were observed by electronic microscopy, proved to be antigenically and immunogenically similar to the virion. Indeed, anti-IBDV antibodies from samples of infected birds recognized these SVP and, when injected intramuscularly, these subviral particles also evoked a humoral immune response in chickens. We developed an in-house ELISA using SVP as coating reagent that demonstrated to be highly accurate and in good agreement with a commercial ELISA. This study demonstrates that the recombinant antigen generated and the technology used to produce it are suitable for developing a diagnostic tool against Infectious bursal disease.
传染性法氏囊病病毒（IBDV）是一种影响幼禽的免疫抑制性和高度传染性疾病的病原体，因此给家禽业造成了重大经济损失。基于衣壳蛋白VP2的具有不同结构的多聚体颗粒已被广泛生产用于不同目的。我们在此展示了从瞬时转化的本氏烟草中生产和轻松回收IBDV亚病毒颗粒（SVP）的方法。通过电子显微镜观察到的SVP在抗原性和免疫原性上被证明与病毒粒子相似。事实上，来自感染鸟类样本的抗IBDV抗体识别这些SVP，并且当肌肉注射时，这些亚病毒颗粒也在鸡中引发了体液免疫反应。我们开发了一种使用SVP作为包被试剂的内部ELISA，其被证明具有高度准确性，并且与商业ELISA结果高度一致。这项研究表明，所产生的重组抗原及其生产技术适用于开发针对传染性法氏囊病的诊断工具。
