Alcain F J
Departamento de Ciencias Morfológicas, Facultad de Veterinaria, Universidad de Córdoba, Spain.
Int J Neurosci. 1988 Dec;43(3-4):189-93. doi: 10.3109/00207458808986169.
Neurons from ciliary ganglia (CG) from 8 to 14 day-old chick embryos were cultured in presence of conditioned media (CM) by eye tissue cells (ETC) on nonneuronal cells from ciliary ganglia (NFGC). These conditioning cells were obtained from 8 and 14 day-old embryos. Two parameters, surviving neurons and neurons displaying neurites, were determined after 48 h of culture. For neuronal survival, CMs did not show an effect on CG14 neurons. In the other neuronal ages ETC-CMs maintained a similar neuronal survival, whereas NFGC-CMs were more effective on older neurons. CM14 media were more effective maintaining neuronal survival than CM8 media respectives. The number of neurons displaying neurites decreased with neuronal ages in presence of all CMs. ETC8-CM was the better promoting neurite extension in all neuronal ages tested.
将8至14日龄鸡胚的睫状神经节（CG）神经元，在由眼组织细胞（ETC）产生的条件培养基（CM）存在的情况下，培养于来自睫状神经节的非神经元细胞（NFGC）上。这些条件细胞取自8日龄和14日龄胚胎。培养48小时后，测定两个参数，即存活神经元和显示出神经突的神经元。对于神经元存活，CM对CG14神经元没有影响。在其他神经元年龄组中，ETC-CM维持相似的神经元存活，而NFGC-CM对较老的神经元更有效。CM14培养基在维持神经元存活方面比CM8培养基更有效。在所有CM存在的情况下，显示神经突的神经元数量随神经元年龄增加而减少。在所有测试的神经元年龄组中，ETC8-CM在促进神经突延伸方面效果最佳。
