通过制备型高效液相色谱法从胸腺素组分5中快速分离胸腺素β4。

Rapid isolation of thymosin beta 4 from thymosin fraction 5 by preparative high-performance liquid chromatography.

作者信息

Badamchian M, Strickler M P, Stone M J, Goldstein A L

机构信息

Department of Biochemistry, George Washington University, School of Medicine, Washington, DC 20037.

出版信息

J Chromatogr. 1988 Dec 28;459:291-300. doi: 10.1016/s0021-9673(01)82039-2.

DOI:10.1016/s0021-9673(01)82039-2

PMID:3243904

Abstract

We have developed a rapid, efficient, and reproducible two-step method for the purification of thymosin beta 4 (T beta 4) from thymosin fraction 5 (TF5). This purification is based on the use of high-performance preparative/semi-preparative and analytical reversed-phase (Delta-Pak C18) chromatographic columns. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and amino acid compositional analysis have shown that natural, purified T beta 4 is identical to synthetic T beta 4. This procedure can be used to isolate other biologically active peptides from TF5 in sufficient quantity for characterization.

摘要

我们开发了一种快速、高效且可重复的两步法，用于从胸腺素组分5（TF5）中纯化胸腺素β4（Tβ4）。这种纯化方法基于使用高性能制备/半制备和分析型反相（Delta-Pak C18）色谱柱。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和氨基酸组成分析表明，天然纯化的Tβ4与合成的Tβ4相同。该方法可用于从TF5中分离出足够量的其他生物活性肽以进行表征。