Kasama T, Tanaka H
Division of Mental Retardation and Birth Defect Research, National Institute of Neuroscience, Tokyo, Japan.
J Nutr Sci Vitaminol (Tokyo). 1988 Dec;34(6):595-605. doi: 10.3177/jnsv.34.595.
The effects of copper administration to neonatal male mice on the copper concentrations and activities of copper-containing enzymes in cerebrum, liver, and kidney were studied. Intraperitoneal copper injections at 7 and 10 days of age increased the activities of superoxide dismutase and cytochrome oxidase in cerebrum and liver, and also increased the copper concentrations in cerebrum, liver, and kidney at 13 days of age. Maternal copper administration during the late-gestational period (from 13 days gestation to delivery) decreased the activities of both enzymes and increased the copper concentration in cerebrum. This increased level of copper remained by 13 days of age after birth. Liver showed similar changes to those in cerebrum, but the renal responses were less remarkable. Maternal copper administration from the late-gestational through lactational periods affected neonatal growth, decreased the activity of cytochrome oxidase, and increased the copper concentrations in all tissues examined. It is known that the copper concentration and copper-containing enzyme activity are low in cerebrum of mottled mice as well as of patients with Menkes' disease. These results suggested that the cytochrome oxidase activity in cerebrum was decreased by not only copper deficiency but also excess. The combination of prenatal copper supplementation by means of maternal copper administration during the late-gestational period and intraperitoneal copper injections after birth, while being careful not to overdose, is expected to be efficient for the copper supplementation to mottled mice.
研究了给新生雄性小鼠注射铜对其大脑、肝脏和肾脏中铜浓度及含铜酶活性的影响。在7日龄和10日龄时腹腔注射铜，可增加大脑和肝脏中超氧化物歧化酶和细胞色素氧化酶的活性，并且在13日龄时还可增加大脑、肝脏和肾脏中的铜浓度。在妊娠后期（从妊娠第13天至分娩）给母鼠补充铜，会降低这两种酶的活性，并增加大脑中的铜浓度。出生后13日龄时，这种升高的铜水平依然存在。肝脏呈现出与大脑类似的变化，但肾脏的反应不太明显。从妊娠后期到哺乳期给母鼠补充铜会影响新生小鼠的生长，降低细胞色素氧化酶的活性，并增加所有检测组织中的铜浓度。已知斑驳小鼠以及患有门克斯病的患者大脑中的铜浓度和含铜酶活性较低。这些结果表明，大脑中的细胞色素氧化酶活性不仅会因铜缺乏而降低，也会因铜过量而降低。在妊娠后期通过给母鼠补充铜进行产前补铜，并在出生后进行腹腔注射铜，同时注意不过量，预计这种方法对斑驳小鼠补铜有效。
