College of Oceanology and Food Science, Quanzhou Normal University/Key Laboratory of Inshore Resources Biotechnology, Quanzhou, 362000, China; School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212001, PR China.
Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, 50 Zhongling Street, Nanjing, 210014, China.
Chemosphere. 2020 Aug;252:126640. doi: 10.1016/j.chemosphere.2020.126640. Epub 2020 Mar 30.
This work focuses on the bioaccumulation and toxic effects of di-(2-ethylhexyl) phthalate (DEHP) in the leafy vegetable Shanghaiqing (SHQ) (Brassica chinensis L.). The accumulated DEHP amount in the edible part and roots of SHQ increased as the DEHP concentration in the soil increased. DEHP accumulation was higher in the roots than in the edible part of the plant. The root concentration factors and bioaccumulation factors for DEHP in SHQ were 0.13-2.49 and 0.03-2.00, respectively. The DEHP translocation factors were below 1.0, indicating that DEHP preferentially accumulated in plant roots. The DEHP risk index in the edible part of SHQ in relation to the human body and in terms of dietary exposure risk assessment was also below 1.0, indicating a low health risk. High DEHP concentrations caused 1) inhibition of SHQ growth, 2) an increase in SHQ chlorophyll and malondialdehyde contents and 3) a decrease in soluble sugar and vitamin contents. Low DEHP concentrations stimulated total superoxide dismutase, peroxidase and catalase activities, while high DEHP levels showed an inhibitory effect. DEHP presence in soil affected not only SHQ growth but also quality. Our results provide the data needed for the proper assessment of food safety and the ecological impact of DEHP contamination in agricultural soils.
本研究聚焦于邻苯二甲酸二（2-乙基己基）酯（DEHP）在叶菜上海青（Brassica chinensis L.）中的生物累积和毒性效应。随着土壤中 DEHP 浓度的增加，上海青可食用部分和根部的 DEHP 累积量增加。DEHP 在植物根部的累积量高于可食用部分。上海青中 DEHP 的根浓度系数和生物累积系数分别为 0.13-2.49 和 0.03-2.00。DEHP 的迁移系数均小于 1.0，表明 DEHP 优先在植物根部累积。从人体健康和膳食暴露风险评估角度来看，上海青可食用部分的 DEHP 风险指数均低于 1.0，表明健康风险较低。高浓度的 DEHP 会导致 1）上海青生长受到抑制，2）上海青叶绿素和丙二醛含量增加，3）可溶性糖和维生素含量降低。低浓度的 DEHP 会刺激总超氧化物歧化酶、过氧化物酶和过氧化氢酶的活性，而高浓度的 DEHP 则表现出抑制作用。土壤中 DEHP 的存在不仅影响上海青的生长，还影响其品质。我们的研究结果为评估食品安全和农业土壤中 DEHP 污染的生态影响提供了必要的数据。
