一种用于定量检测有毒醇类的单柱气相色谱法。

A Single-Column Gas Chromatography Method for Quantifying Toxic Alcohols.

机构信息

Department of Pathology and Laboratory Medicine, Dartmouth-Hitchcock Medical Center, Lebanon, NH; The Geisel School of Medicine at Dartmouth, Hanover, NH.

出版信息

J Appl Lab Med. 2020 Mar 1;5(2):300-310. doi: 10.1093/jalm/jfz019.

DOI:10.1093/jalm/jfz019

PMID:32445388

Abstract

BACKGROUND

Rapid identification and quantification of toxic alcohols and ethylene glycol is imperative for appropriate treatment. Clinical laboratories frequently rely on direct injection gas chromatography (GC) methods, but these methods require inlet maintenance and multiple GC systems. To overcome these challenges, we developed a single-column headspace GC method for both toxic alcohols and glycols that streamlines patient sample analysis for toxic alcohol ingestion.

METHODS

Optimal parameters for nonderivatized (volatile) and derivatized (glycol) plasma samples were determined using a 7890 A headspace sampler, an Agilent 7697 A GC system, a DB-200 column, and a flame ionization detector. Limit of Quantification (LoQ), linearity, imprecision, carry-over, method comparison, and interference studies were performed using quality control materials and prepared plasma samples.

RESULTS

Our volatile method is linear to 3000 mg/L (ethanol) with LoQ concentrations below 20 mg/L (ethanol). The glycol method is linear to 2000 mg/L (ethylene glycol) with LoQ concentrations below 40 mg/L (ethylene glycol). Total assay impression ranged from 1.7% for ethanol to 13.3% for propylene glycol. Both methods were free of sample carryover and compared favorably with a similar clinical method at an outside laboratory. Propionic acid, an accumulating metabolite in methylmalonic acidemia that interferes with ethylene glycol identification by a different method, did not interfere with the ethylene glycol method reported here.

CONCLUSIONS

Our single-column headspace GC method provides reliable, robust, and rapid identification and quantification of commonly encountered toxic alcohols. Clinical laboratories relying on direct injection Gas Chromatography (GC) for toxic alcohol analysis face challenges including frequent inlet maintenance, sample carryover, or the need for separate GC systems for volatile and glycol analysis. We summarize our development and optimization of two headspace GC methods for nonderivatized (volatile) and derivatized (glycol) plasma samples that use a single DB-200 analytical column. These methods are comparable to other GC methods, not prone to sample carryover, eliminate the need for multiple GC systems or columns, and are readily applicable to other laboratories that provide toxic alcohol analysis.

摘要

背景

快速识别和定量有毒醇和乙二醇对于适当的治疗至关重要。临床实验室通常依赖于直接进样气相色谱（GC）方法，但这些方法需要进样口维护和多个 GC 系统。为了克服这些挑战，我们开发了一种用于有毒醇和乙二醇的单柱顶空 GC 方法，简化了有毒醇摄入患者样本的分析。

方法

使用 7890A 顶空进样器、Agilent 7697A GC 系统、DB-200 柱和火焰电离检测器，确定非衍生（挥发性）和衍生（乙二醇）血浆样品的最佳参数。使用质控材料和制备的血浆样品进行定量限（LoQ）、线性、精密度、携带污染、方法比较和干扰研究。

结果

我们的挥发性方法在 3000mg/L（乙醇）范围内呈线性，定量限浓度低于 20mg/L（乙醇）。乙二醇方法在 2000mg/L（乙二醇）范围内呈线性，定量限浓度低于 40mg/L（乙二醇）。总分析印象范围从乙醇的 1.7%到丙二醇的 13.3%。两种方法均无样品携带污染，与外部实验室的类似临床方法相比表现良好。丙酸是甲基丙二酸血症中积累的代谢物，会干扰其他方法鉴定乙二醇，但不会干扰本文报道的乙二醇方法。

结论

我们的单柱顶空 GC 方法可可靠、稳健、快速地鉴定和定量常见的有毒醇。依赖直接进样气相色谱（GC）分析有毒醇的临床实验室面临着一些挑战，包括频繁的进样口维护、样品携带污染或需要单独的 GC 系统用于挥发性和乙二醇分析。我们总结了我们为非衍生（挥发性）和衍生（乙二醇）血浆样品开发和优化的两种顶空 GC 方法，这些方法使用单个 DB-200 分析柱。这些方法与其他 GC 方法相当，不易发生样品携带污染，消除了对多个 GC 系统或柱子的需求，并且易于应用于提供有毒醇分析的其他实验室。