Department of Pediatric Surgery, Affiliated Hospital of North Sichuan Medical College, Nanchong, China; Department of Clinical Medicine, North Sichuan Medical College, Nanchong, China.
Department of Medical Laboratory, the First Affiliated Hospital of Chengdu Medical College, Chengdu, China.
Exp Mol Pathol. 2020 Aug;115:104469. doi: 10.1016/j.yexmp.2020.104469. Epub 2020 May 21.
To uncover the expression patterns of HOXB2 and FOXC1 in Wilms tumor samples, and their synergistical regulations on the development of Wilms tumor.
Expression levels of HOXB2 and FOXC1 in 58 cases of Wilms tumor tissues and paracancerous ones were detected. The influences of HOXB2 and FOXC1 on prognosis in Wilms tumor patients were analyzed. Their regulatory effects on proliferative and migratory abilities in WT-CLS1 and HFWT cells were examined by cell counting kit-8 (CCK-8) and Transwell assay, respectively. The interaction between HOXB2 and FOXC1, and their synergistical regulation on the development of Wilms tumor were finally explored.
HOXB2 and FOXC1 were upregulated in Wilms tumor tissues. Higher levels of HOXB2 and FOXC1 indicated higher risks of advanced stage and lymphatic metastasis, as well as worse prognosis in Wilms tumor patients. Knockdown of HOXB2 or FOXC1 weakened proliferative and migratory abilities in WT-CLS1 and HFWT cells, while the opposite trends were observed in those overexpressing HOXB2 or FOXC1. The positive interaction between HOXB2 and FOXC1 was identified, which synergistically drove the malignant development of Wilms tumor.
HOXB2 and FOXC1 are upregulated in Wilms tumor samples, and they are closely linked to tumor staging and lymphatic metastasis in Wilms tumor patients. HOXB2 and FOXC1 synergistically drive the malignant development of Wilms tumor by stimulating proliferative and migratory potentials.
揭示 HOXB2 和 FOXC1 在威尔姆斯瘤组织样本中的表达模式，以及它们在威尔姆斯瘤发生发展中的协同调控作用。
检测 58 例威尔姆斯瘤组织及其癌旁组织中 HOXB2 和 FOXC1 的表达水平。分析 HOXB2 和 FOXC1 对威尔姆斯瘤患者预后的影响。通过细胞计数试剂盒-8（CCK-8）和 Transwell 分析分别检测 HOXB2 和 FOXC1 对 WT-CLS1 和 HFWT 细胞增殖和迁移能力的影响。最后，探讨 HOXB2 和 FOXC1 之间的相互作用及其对威尔姆斯瘤发生发展的协同调控作用。
HOXB2 和 FOXC1 在威尔姆斯瘤组织中上调。HOXB2 和 FOXC1 水平较高提示威尔姆斯瘤患者处于晚期和淋巴转移风险较高，预后较差。敲低 HOXB2 或 FOXC1 可减弱 WT-CLS1 和 HFWT 细胞的增殖和迁移能力，而过表达 HOXB2 或 FOXC1 则呈现相反的趋势。鉴定出 HOXB2 和 FOXC1 之间存在正相互作用，协同驱动威尔姆斯瘤的恶性发展。
HOXB2 和 FOXC1 在威尔姆斯瘤样本中上调，与威尔姆斯瘤患者的肿瘤分期和淋巴转移密切相关。HOXB2 和 FOXC1 通过刺激增殖和迁移潜能协同驱动威尔姆斯瘤的恶性发展。
