Gupta S K, Ellinwood E H
Department of Psychiatry, Duke University Medical Center, Durham, North Carolina 27710.
Pharm Res. 1988 Jun;5(6):365-8. doi: 10.1023/a:1015907611170.
A reverse-phase liquid chromatographic method is described for simultaneous quantification of quazepam, and two of its metabolites, 2-oxoquazepam and N-desaklyl-2-oxoquazepam. The method uses a solid-phase extraction procedure to prepare plasma samples. After extraction, the methanolic extract is evaporated; the residue is then reconstituted in a small volume of mobile phase and chromatographed. The total chromatography time for a single sample is about 20 min. A sensitivity of 1 ng/ml for quazepam and its metabolites is attained when 1 ml of plasma is extracted. Analytical recovery of quazepam and its metabolites added to plasma ranged from 87 to 96%. The maximum within-day and day-to-day coefficients of variation for each compound at concentrations of 20 and 60 ng/ml were 7.6 and 11.2%, respectively. The method was applied to sublingual pharmacokinetic studies of quazepam in healthy volunteers.
描述了一种反相液相色谱法，用于同时定量地西泮及其两种代谢物，即2-氧代地西泮和N-去烷基-2-氧代地西泮。该方法采用固相萃取程序制备血浆样品。萃取后，将甲醇提取物蒸发；然后将残留物用少量流动相复溶并进行色谱分析。单个样品的总色谱分析时间约为20分钟。当提取1 ml血浆时，地西泮及其代谢物的灵敏度达到1 ng/ml。添加到血浆中的地西泮及其代谢物的分析回收率在87%至96%之间。每种化合物在浓度为20和60 ng/ml时，日内和日间最大变异系数分别为7.6%和11.2%。该方法应用于健康志愿者中地西泮的舌下给药药代动力学研究。
