Semple H A, Tam Y K, Tin S, Coutts R T
Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Canada.
Pharm Res. 1988 Jun;5(6):383-6. doi: 10.1023/a:1015915812987.
A new method of analysis for the antihypertensive drug, hydralazine, is introduced. The assay involves the addition of p-nitrobenzaldehyde to blood samples containing hydralazine, to form a stable Schiff's base, hydralazine p-nitrobenzaldehyde hydrazone. The derivative is extracted from the blood into hexane and the samples are dried under a nitrogen stream. The extracts are then dissolved in mobile phase and analyzed using high-performance liquid chromatography. The extracted samples can be stored for at least 7 days at room temperature or at -20 degrees C. The sensitivity of the assay is better than 300 pg/ml using 3-ml blood samples, and the range can extend to 640 ng/ml. The stability of the extracted samples plus the sensitivity and simplicity of the assay are the main advantages of the method over other selective methods for hydralazine.
介绍了一种分析抗高血压药物肼屈嗪的新方法。该测定方法包括向含有肼屈嗪的血样中加入对硝基苯甲醛，以形成稳定的席夫碱——肼屈嗪对硝基苯甲醛腙。该衍生物从血液中萃取到己烷中，样品在氮气流下干燥。然后将提取物溶解在流动相中，并用高效液相色谱法进行分析。萃取后的样品在室温或 -20℃下可保存至少7天。使用3毫升血样时，该测定方法的灵敏度优于300 pg/ml，范围可扩展至640 ng/ml。与其他检测肼屈嗪的选择性方法相比，该方法的主要优点在于萃取后样品的稳定性以及测定的灵敏度和简便性。
