CEB - Centre of Biological Engineering, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal.
CICECO-Aveiro Institute of Materials and Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
Bioresour Technol. 2020 Sep;311:123524. doi: 10.1016/j.biortech.2020.123524. Epub 2020 May 12.
The aim of this study was to evaluate the effect of aqueous solutions of deep eutectic solvent, Cholinium Chloride:Urea ([Ch]Cl:U) at 50 wt% and 20 wt%, using different molar ratios (1:1, 2:1 and 1:2) on the enzymatic hydrolysis of xylan for xylose production and its subsequent bioconversion into xylitol using a recombinant yeast strain. The lowest xylan conversion into xylose (45%) was obtained using 1:2 [Ch]Cl:U molar ratio. On the other hand, the 1:1 [Ch]Cl:U molar ratio, at 20 wt% in water, improved this conversion, achieving the highest xylose yield (81.4%). The xylitol production was then optimized with [Ch]Cl:U (1:1) at 20 wt% by simultaneous saccharification and fermentation process, attaining 23.67 g/L, corresponding to 66.04% of xylitol yield. This study reveals the possibility of using xylan solubilized in DES aqueous solutions directly for xylitol production, thus assembling a one-step process.
本研究旨在评估不同摩尔比（1:1、2:1 和 1:2）的 50wt%和 20wt%深共熔溶剂水合氯化胆碱-尿素 ([Ch]Cl:U) 溶液对木聚糖酶解生成木糖及其后续利用重组酵母菌株转化为木糖醇的影响。使用 1:2 [Ch]Cl:U 摩尔比时，木聚糖转化为木糖的转化率最低（45%）。另一方面，在水中 20wt%的 1:1 [Ch]Cl:U 摩尔比提高了转化率，达到了最高的木糖得率（81.4%）。然后通过同步糖化发酵工艺对 [Ch]Cl:U（1:1）在 20wt%时进行了木糖醇生产的优化，获得了 23.67g/L 的产量，对应的木糖醇得率为 66.04%。本研究揭示了直接使用 DES 水溶液中溶解的木聚糖生产木糖醇的可能性，从而组装了一个一步法工艺。
