College of Plant Science, Jilin University, Changchun 130062, PR China; School of Agricultural Science, Zhengzhou University, Zhengzhou 450001, PR China.
College of Plant Science, Jilin University, Changchun 130062, PR China.
Pestic Biochem Physiol. 2020 Jun;166:104565. doi: 10.1016/j.pestbp.2020.104565. Epub 2020 Mar 23.
Uridine diphosphate (UDP)-glycosyltransferases (UGTs) catalyze the conjugation of small lipophilic endogenous and exogenous compounds with sugars to produce water-soluble glycosides, playing an important role in insect endobiotic regulation and xenobiotic detoxification. In this study, two UGT-inhibitors, sulfinpyrazone and 5-nitrouracil, significantly increased spirotetramat toxicity against third instar nymphs of resistant Aphis gossypii, whereas there were no synergistic effects in apterous adult aphids, suggesting UGT involvement in spirotetramat resistance in cotton aphids. Furthermore, the UHPLC-MS/MS was employed to determine the content of spirotetramat and its four metabolites (S-enol, S-glu, S-mono, S-keto) in the honeydew of resistant cotton aphids under spirotetramat treatment. No residual spirotetramat was detected in the honeydew, while its four metabolites were detected at a S-enol: S-glu: S-mono: S-keto ratio of 69.30: 6.54: 1.44: 1.00. Therefore, glycoxidation plays a major role in spirotetramat inactivation and excretion in resistant aphids. Compared with the susceptible strain, the transcriptional levels of UGT344M2 were significantly upregulated in nymphs and adults of the resistant strain. RNA interference of UGT344M2 dramatically increased spirotetramat toxicity in nymphs, but no such effect were found in the resistant adult aphids. Overall, UGT-mediated glycoxidation were found to be involved in spirotetramat resistance. The suppression of UGT344M2 significantly increased the sensitivity of resistant nymphs to spirotetramat, suggesting that UGT344M2 upregulation might be associated with spirotetramat detoxification. This study provides an overview of the involvement of metabolic factors, UGTs, in the development of spirotetramat resistance.
尿苷二磷酸（UDP）-糖基转移酶（UGTs）催化小亲脂性内源性和外源性化合物与糖的缀合，产生水溶性糖苷，在昆虫内源性调节和外源性解毒中发挥重要作用。在这项研究中，两种 UGT 抑制剂，磺吡酮和 5-硝基尿嘧啶，显著增加了螺虫乙酯对抗药性棉蚜三龄若虫的毒性，而在无翅成蚜中没有协同作用，表明 UGT 参与了棉蚜对螺虫乙酯的抗性。此外，采用 UHPLC-MS/MS 测定了螺虫乙酯及其四种代谢物（S-烯醇、S-葡糖、S-单、S-酮）在抗药性棉蚜蜜露中的含量。在蜜露中未检测到残留的螺虫乙酯，而其四种代谢物的检测比例为 S-烯醇：S-葡糖：S-单：S-酮为 69.30:6.54:1.44:1.00。因此，糖基化在抗药性蚜虫中螺虫乙酯的失活和排泄中起主要作用。与敏感品系相比，抗性品系的若虫和成虫中 UGT344M2 的转录水平显著上调。UGT344M2 的 RNA 干扰显著增加了若虫对螺虫乙酯的毒性，但在抗性成蚜中没有发现这种作用。总的来说，UGT 介导的糖基化被发现参与了螺虫乙酯的抗性。UGT344M2 的抑制显著增加了抗性若虫对螺虫乙酯的敏感性，表明 UGT344M2 的上调可能与螺虫乙酯的解毒有关。本研究概述了代谢因子 UGTs 在螺虫乙酯抗性发展中的作用。
