Department of Endocrinology, Beijing Tongren Hospital, Capital Medical University, 1 Dong Jiao Min Xiang, Beijing, 100730, China.
Department of Mathematics, School of Biomedical Engineering, Capital Medical University, Beijing, 100069, China.
J Endocrinol Invest. 2021 Feb;44(2):297-310. doi: 10.1007/s40618-020-01298-2. Epub 2020 May 24.
A previous study indicated that gut microbiota changed notably in Graves' orbitopathy (GO) patients as compared to controls. However, the characteristics of intestinal bacteria in Graves' disease (GD) and GO are unclear.
The present study aimed to identify specific intestinal bacteria of GD and GO, respectively.
The gut microbial communities of the fecal samples of 30 GD patients without GO, 33 GO subjects, and 32 healthy subjects were analyzed and compared by 16S rRNA gene sequencing.
At the phylum level, the proportion of Deinococcus-Thermus and Chloroflexi was decreased significantly in GO patients as compared to GD. At the genus level, the proportion of Subdoligranulum and Bilophila was increased while that of Blautia, Anaerostipes, Dorea, Butyricicoccus, Romboutsia, Fusicatenibacter, unidentified_ Lachnospiraceae, unidentified_Clostridiales, Collineslla, Intestinibacter, and Phascolarctobacterium was decreased in the GO group as compared to the GD group. Random forest analysis was used for the identification of specific intestinal microbiota, and Deinococcus-Thermus, Cyanobacteria and Chloroflexi were ranked in the top ten according to their contributions to sample classification. Moreover, compared to the control, there were multiple gut bacterial enrichment metabolic pathways in GO and GD patients, including nucleotide metabolism, enzyme family, and energy metabolism. Compared to GO, the only enrichment metabolic pathway found in GD was the viral protein family.
This study highlighted the significant differences in the intestinal microbiota and predictive functions of GD with GO, thereby providing new insights into the role of the gut bacteria that might contribute to the development of GO in GD patients.
先前的研究表明，与对照组相比，格雷夫斯眼病（GO）患者的肠道微生物群有明显变化。然而，格雷夫斯病（GD）和 GO 患者肠道细菌的特征尚不清楚。
本研究旨在分别鉴定 GD 和 GO 的特定肠道细菌。
通过 16S rRNA 基因测序分析并比较了 30 名无 GO 的 GD 患者、33 名 GO 患者和 32 名健康对照者的粪便样本肠道微生物群落。
在门水平上，GO 患者肠道中 Deinococcus-Thermus 和 Chloroflexi 的比例显著降低。在属水平上，与 GD 组相比，GO 组 Subdoligranulum 和 Bilophila 的比例增加，而 Blautia、Anaerostipes、Dorea、Butyricicoccus、Romboutsia、Fusicatenibacter、未鉴定的 Lachnospiraceae、未鉴定的 Clostridiales、Collinesella、Intestinibacter 和 Phascolarctobacterium 的比例降低。随机森林分析用于鉴定特定的肠道微生物群，根据对样本分类的贡献，Deinococcus-Thermus、Cyanobacteria 和 Chloroflexi 排名前十。此外，与对照组相比，GO 和 GD 患者存在多个肠道细菌富集代谢途径，包括核苷酸代谢、酶家族和能量代谢。与 GO 相比，GD 患者唯一富集的代谢途径是病毒蛋白家族。
本研究强调了 GD 伴 GO 患者肠道微生物群和预测功能的显著差异，从而为肠道细菌在 GD 患者 GO 发展中的作用提供了新的见解。
